KANSAS
STATE UNIVERSITY
BIOTECHNOLOGY CORE LAB
John
M. Tomich - Ph.D. - Director
Takeo
Iwamoto - Ph.D. - Associate Director
Yasuaki Hiromasa
- Ph.D. - Research Associate
Burt Hall, Rooms 201, 207, 211, 216
Telephone (785) 532-5956
Fax (785) 532-6297
E-mail: jtomich@ksu.edu
| The Biotechnology Core Facility at Kansas State
University was established in 1993 to provide a number of centralized
services to plant and animal researchers at K-State and elsewhere. The
goods and services provided by the facility give researchers the tools
they need to identify new proteins, protein modifications,
protein-protein interactions and enzyme substrates. The lab is located
in space provided by the department of biochemistry and the facility is
supported in part by fees for service and the K-State Colleges of
Agriculture and Arts and Sciences. The laboratory functions in three
distinct ways: synthesis, separations and bioanalysis. The lab
synthesizes peptides, peptide libraries, chromophores, fluorophores and
other organic molecules. It also serves as a broker for ordering
oligonucleotides. Separations are performed using various
chromatographic techniques and by mass spectrometry. Bioanalysis is
performed to assess purity, size, composition and sequences of
peptides, proteins and carbohydrates. The facility is equipped with more than $3 million worth of automated scientific instruments that enhance the biotechnology capabilities of the university. One piece of equipment, the Biacore, gives the core facility a new dimension, the quantification of molecular interactions. This instrument uses a treated gold slide that allows the covalent attachment and display of molecules, such as proteins or lipids, in a flow cell. Potential binding partners can be introduced in solution and subsequent binding to the bound molecule can be measured. Using this technique, accurate association constants can be determined.
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This page last updated: May 19, 2006