Protocols and Methodology

Listed below are protocols related to lipid profiling. These methods have been optimized for lipidomics analyses.

• Extraction Method for Arabidopsis and Other Leaves for Lipid Profiling (3 methods - NEW: Videos for Methods 1 and 2)
• Extraction Method for Arabidopsis Seeds for Lipid Profiling
• Extraction Method for Animal Tissue for Lipid Profiling
• Extraction Method for Yeast for Lipid Profiling
• Q-Test

Acquisition methods for the KLRC's mass spectrometer set-ups are available:

• Acquisition methods

Instrument acquisition and lipidomics method development were supported by the National Science Foundation (including support from the Major Research Instrumentation program; most recent award DBI-2511193), USDA National Institute of Food and Agriculture (Hatch/Multi-State project 7001195), and Kansas State University. Any opinions, findings, and conclusions or recommendations expressed in this web material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation or other granting agencies.