Facility Services
Currently, we provide services on DNA and RNA quality control, NGS library preparation, MiSeq, MiSeq i100 Plus and PacBio based sequencing, single cell sequencing, and BD FACS Melody cell sorting.
Next Generation Sequencing (NGS) Illumina Library Prep
We perform Illumina NGS library preparation from a variety of starting materials, including genomic DNA, PCR products, total RNA, and mRNA. These libraries can be sequenced on various Illumina platforms, such as the MiSeq and MiSeq i100 Plus Personal Systems, NextSeq 2000, NovaSeq X, and others. Applications include de novo sequencing, whole-genome and targeted DNA resequencing, sequencing of individual chloroplast genomes, metagenomic analysis of environmental samples, RNA profiling, and more.
Illumina Sequencing
Illumina Sequencing Technology, sequencing by synthesis (SBS), is a widely adopted next-generation sequencing (NGS) technology worldwide.
DNA, RNA, NGS library Quantification and Quality Control
The Agilent 2100 Bioanalyzer and 4150 Tape Station are sensitive automated electrophoresis platforms designed for the analysis of DNA and RNA samples using nano- or picogram amounts of material. The Tape Station’s software allows for fast and intuitive sample analysis (concentration, size, and integrity) with different visualization options and analysis tools including the calculation of the DNA and RNA Integrity Numbers (DIN and RIN), which are objective quality metrics for DNA and RNA samples.
We recommend qubit-based quantification of DNA and RNA samples.
DNA Fragmentation
The Covaris is based on Adaptive Focused Acoustics (AFA) technology and the technology yields precise and tight fragment size distribution.
Cell Sorting
Our newest piece of equipment is the BD FACS Melody system.