Yeast can be extracted by a method similar to the one used for "Extraction of lipids from animal
tissue," but usually it is necessary to apply some force to break the cells. One possibility is sonication. Another is use of a Potter-Elvehejem (Teflon-glass; driven by a drill motor or similar) homogenizer after addition of the solvent to the sample.
Safety tip:
Wear safety glasses and protective clothing,
especially when operating a Potter-Elvehejem homogenizer with the sample in solvent,
because the solvent can accidentally splatter. To avoid this, start slowly.
When extraction is complete: Fill
tubes with nitrogen, store in freezer; and transport to the KLRC
Analytical Laboratory on dry ice. Alternatively, if you need to ship
the samples, evaporate the solvent, fill the tubes to nitrogen, and
ship on dry ice. You may wish to consider transferring the samples
to 2 ml vials with teflon-lined caps for shipping. Please contact
Mary Roth at mrroth@ksu.edu
before shipping. |
