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Applied Biosystems
ESI-MS/MS
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Extraction of lipids from yeast

 

Yeast can be extracted by a method similar to the one used for "Extraction of lipids from animal tissue," but usually it is necessary to apply some force to break the cells. One possibility is sonication.  Another is use of a Potter-Elvehejem (Teflon-glass; driven by a drill motor or similar) homogenizer after addition of the solvent to the sample.

Safety tip:

Wear safety glasses and protective clothing, especially when operating a Potter-Elvehejem homogenizer with the sample in solvent, because the solvent can accidentally splatter.  To avoid this, start slowly.

 

When extraction is complete: Fill tubes with nitrogen, store in freezer; and transport to the KLRC Analytical Laboratory on dry ice. Alternatively, if you need to ship the samples, evaporate the solvent, fill the tubes to nitrogen, and ship on dry ice.  You may wish to consider transferring the samples to 2 ml vials with teflon-lined caps for shipping. Please contact Mary Roth at mrroth@ksu.edu before shipping.

 Copyright 2005-2007 @ Kansas Lipidomics Research Center,Kansas State University.
 Website  design by Prashanth Palakollu.

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Page last updated July 3, 2007

 Copyright 2005 @ Kansas Lipidomcs Research Center , Kansas State University.Website  designed by Prashanth Palakollu.