The Kansas Lipidomics Research Center's Analytical Laboratory has two electrospray ionization triple quadrupole mass spectrometers (Applied Biosystems API 4000 and Applied Biosystems Q-TRAP). We also have a chip-based nanoelectrospray source (Advion Nanomate) that makes analysis of small samples entirely practicable. These instruments can also be interfaced with autosamplers. In addition, we have an electrospray ionization quadrupole/time-of-flight mass spectrometer (Applied Biosystems Q_STAR Elite) for accurate mass analysis and two gas chromatograph/mass spectrometers (Agilent, with chemical ionization and electron impact). We offer characterization and quantitation of phospholipid and galactoglycerolipid molecular species (see species analyzed
) from crude solvent extracts of living tissues. We are currently prepared to analyze the major lipid species from plant
, and animal
ESI results in the generation of singly charged molecular ions with excellent concentration sensitivity, while an MS/MS precursor scan allows detection of all species within a lipid head group class. Sequential scanning of the crude extracts in various MS/MS precursor scan modes gives a complete profile of the molecular species in many lipid classes, with speciation to the level of total acyl chain length and total number of double bonds. A more detailed explanation is available.