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Analytical Laboratory
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Kartik, doctoral student

Analytical Laboratory

The Kansas Lipidomics Research Center's Analytical Laboratory has two electrospray ionization triple quadrupole mass spectrometers (Applied Biosystems API 4000 and Applied Biosystems Q-TRAP).  We also have a chip-based nanoelectrospray source (Advion Nanomate) that makes analysis of small samples entirely practicable.  These instruments can also be interfaced with autosamplers.  In addition, we have an electrospray ionization quadrupole/time-of-flight mass spectrometer (Applied Biosystems Q_STAR Elite) for accurate mass analysis and two gas chromatograph/mass spectrometers (Agilent, with chemical ionization and electron impact).  We offer characterization and quantitation of phospholipid and galactoglycerolipid molecular species (see species analyzed) from crude solvent extracts of living tissues.  We are currently prepared to analyze the major lipid species from plant, yeast, and animal samples.

ESI results in the generation of singly charged molecular ions with excellent concentration sensitivity, while an MS/MS precursor scan allows detection of all species within a lipid head group class.  Sequential scanning of the crude extracts in various MS/MS precursor scan modes gives a complete profile of the molecular species in many lipid classes, with speciation to the level of total acyl chain length and total number of double bonds.  A more detailed explanation is available.

Summary of current instumentation:

  • Applied Biosystems 4000 Q-TRAP triple quadrupole mass spectrometer with a linear ion trap and an electrospray source
  • Applied Biosystems API-4000 triple quadrupole mass spectrometer with electrospray and APCI sources
  • Applied Biosystems Q-STAR Elite quadrupole-time-of-flight mass spectrometer with an electrospray source
  • Advion Trivera chip-based nanospray interface that fits the Q-TRAP and Q-STAR
  • 2Agilent Gas Chromatograph/Mass Spectrometers with electron impact and chemical ionization sources (Agilent Technologies 6890N Network GC System/Agilent Technologies 5975 inert XL mass selective detector)

Analytical work includes:

  • Sample preparation from tissue extracts
  • Addition of internal standard mixtures
  • Mass spectrometric analysis
  • Data analysis

Relevant links:

  • Example data generated by lipid profiling.
  • Protocols associated with lipid profiling, including sample preparation.

 Copyright 2005 @ Kansas Lipidomics Research Center , Kansas State University.
 design by Prashanth Palakollu.

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