Instruments Available at the Analytical Laboratory
The Kansas Lipidomics Research Center's Analytical Laboratory has five different mass spectrometer systems which we use to acquire sample data.
Applied Biosystems API-4000(1)
Triple quadrupole mass spectrometer with electrospray and APCI sources. This instrument and the 4000 QTRAP (below) are the workhorses for routine lipid profiling. The "triple quads" have good sensitivity and can provide a linear response as a function of lipid concentration over a large concentration range (100,000 to 1,000,000-fold). Their mass accuracy is "nominal" mass, which mean that the peaks are about 0.7 of a mass unit wide, and typically the absolute value of the mass/charge ratio is within 0.2 units of the actual value.
Applied Biosystems 4000 QTRAP(1)(2)
Triple quadrupole mass spectrometer with a linear ion trap and an
Applied Biosystems QSTAR Elite(2)
Quadrupole-time-of-flight mass spectrometer with an electrospray source. A major use of this instrument is to identify the chemical formulas for lipid fragments, because the “QTOF MS” can detect the mass of a lipid fragments accurately. For example, this instrument can differentiate between a fatty acid that carries an extra oxygen compared to a fatty acid with an odd number of carbons. For example, it can differentiate between fatty acyl anions stemming from hydroxy-18:3 (with a mass/charge ratio of 293.2117) and 19:2 (with a mass/charge ratio of 293.2480).
Waters Xevo TQS(1)
Triple quadrupole mass spectrometer with an electrospray source. This mass spectrometer has similar functionality to the other "triple quads", but greater sensitivity. We currently use it for specialized workflows and a direct infusion plant-lipid MRM method (Vu et al., Plant J. 80, 728).
Agilent Gas Chromatograph/Mass Spectrometer (x 2)
GC/MS with electron impact and chemical ionization sources (Agilent Technologies 6890N Network GC System/Agilent Technologies 5975 inert XL mass selective detector)(3)
This instrument is used for analysis of lipids that can be made volatile. This may involve lipid hydrolysis and typically involves lipid derivatization. Analyses that can be performed by GC/MS include fatty acids, sterols, plant cuticle lipids, and phytohormones.
(1) Note: The Applied Biosystems API-4000, the Applied Biosystems 4000 Q-TRAP, and the Waters Xevo TQS are each interfaced with an auto-sampler, but samples can be manually injected.
(2) Note: Our lab instrumentation also includes an Advion TriVersa NanoMate interface, a chip-based nanoelectrospray source that makes analysis of small samples entirely possible. This instrument fits both our Applied Biosystems 4000 Q-TRAP and Applied Biosystems Q-STAR Elite.
(3) Note: GC with FID is also available.