Department of Biochemistry & Molecular Biophysics
141 Chalmers Hall
Manhattan, KS 66506
785-532-6121
785-532-7278 fax
biochem@k-state.edu



Biotechnology Core Facility
206 Burt Hall
785-532-5956
785-532-6297 fax



Biomolecular NMR Facility
37 Chalmers Hall
785-532-2345

Thomas Roche, Ph.D., University Distinguished Professor

Complete Publications List

2000s | 1990s | 1980s | 1970s | 1960s

2000s

Roche, T.E., Peng, T., Hu, L., Hiromasa, Y., Bao, H., and Gong, X. (2008) Role of Lipoyl domains in the function and Regulation of Mammalian Pyruvate Dehydrogenase Complex. In Lipoic Acid, Energy Production, Antioxidant Activity and Health Effects (Patel, M.S. and Packer, L. Eds) CRC Press, Taylor Francis Group, Boca Raton, pp.167-195.

Hiromasa, Y., Yan, X., and Roche, T. E. (2008) Specific ion influences on self-association of pyruvate dehydrogenase kinase isoform 2 (PDHK2) binding of PDHK2 to the L2 lipoyl domain, and effects of the lipoyl group-binding site inhibitor, Nov3r. Biochemistry 47:2312-2324.

Hiromasa, Y. and Roche, T. E. (2008) Critical role of specific ions for ligand-induced changes regulating pyruvate dehydrogenase kinase isoform 2. Biochemistry 47:2298-2311.

Yu, X., Hiromasa, Y., Tsen, H., Chiu, W., Stoops, J. K., Roche, T. E., and Zhou, Z. H. (2008) Structures of the human pyruvate dehydrogenase complex cores: a highly conserved catalytic center with flexible N-terminal domains. Structure 16:104-114.

Roche, T. E. and Hiromasa, Y. (2007) Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes, heart ischemia, and cancer. Cell. and Mol. Life Sci. 64:830-849.

Knoechel, T. R., Tucker, A. D., Robinson, C. M., Phillips, C., Taylor, W., Bungay, P. J., Kasten, S. A., Roche, T. E., and Brown, D. G. (2006) Regulatory roles of the N-terminal domain based on crystal structures of human pyruvate dehydrogenase kinase 2 containing physiological and synthetic ligands. Biochemistry 45:402-415.

Hiromasa, Y., Hu, L., and Roche, T. E. (2006) Ligand-induced Effects on Pyruvate Dehydrogenase Kinase Isoform 2. J. Biol. Chem. 281:12568-12579.

A. Turkan, Y. Hiromasa and T. E. Roche (2004) Formation of a Complex of the Catalytic Subunit of Pyruvate Dehydrgenase Phospatase Isoform 1 (PDP1c) and the L2 Domain Forms a Ca2+ Binding Site and Captures PDP1c as Monomer. Biochemistry 43, 15073-15085.

H. Bao, S. A. Kasten, X. Yan, Y. Hiromasa, and T. E. Roche (2004) Pyruvate Dehydrogenase Kinase Isoform 2 Activity Stimulated by Speeding Up the Rate of Dissociation of ADP. Biochemistry 43, 13442-13451.

H. Bao, S. A. Kasten, X. Yan, and T. E. Roche (2004) Pyruvate dehydrogenase Kinase Isoform 2 Activity Limited and Further Inhibited by Slowing Down the Rate of Dissociation of ADP, Biochemistry 43, 13432-13441.

Y. Hiromasa, T. Fujisawa, Y. Aso, T. E. Roche (2004) Organization of the Cores of the Mammalian Pyruvate Dehydrogenase Complex Formed by E2 and E2 Plus the E3-binding Protein and Their Capacities to Bind the E1 and E3 Components, J. of Biol. Chemistry. 279, 6921-6933.

J. A. Morell, J. Orme, R. J. Butlin, T. E. Roche, R. M. Mayers, and E. Kilgour (2003) AZD7545 is a selective inhibitor of pyruvate dehydrogenase kinase 2, Biochem. Soc. Trans. 31, 1168-1170.

Y. Hiromasa and T. E. Roche (2003) Facilitated Interaction between the Pyruvate Dehydrogenase Kinase Isoform 2 and the Dihydrolipoyl Acetyltransferase, J. Of Biol. Chemistry. 278, 33681-33693.

T. E. Roche, Y. Hiromasa, A. Turkan, X. Gong, T. Peng, X. Yan, S. A. Kasten, H. Bao and J. Dong (2003) Essential roles of lipoyl domains in the activated function and control of pyruvate dehydrogenase kinases and phosphatase isoform 1, Eur. J. Biochem. 270, 1050-1056.

A. Turkan , X. Gong, T. Peng, and T.E. Roche (2002) Structural Requirements within the Lipoyl Domain for the Ca2+-dependent Binding and Activation of Pyruvate Dehydrogenase Phosphatase Isoform 1 or its Catalytic Subunit, J. Biol. Chem. 277, 14976-14985. [Article]

T. E. Roche, J. Baker, X. Yan, Y. Hiromasa, X. Gong, T. Peng, J. Dong, A. Turkan, and S.A. Kasten (2001) Distinct Regulatory Properties of Pyruvate Dehydrogenase Kinase and Phosphatase Isoforms, Prog. Nucleic Acid Res. Mol. Biol. 70, 33-75.

S. Liu, X. Gong,X. Yan, T. Peng,J. C. Baker, L. Li, P. M. Robben, S. Ravindran, L. A. Andersson, A. B. Cole, and T. E. Roche (2001) Reaction Mechanism for Mammalian Pyruvate Dehydrogenase Using Natural Lipoyl Domain Substrates, Arch. Biochem. Biophys. 386, 123-135. [Article]

M. Sasaki, A. Ansari, N. Pumford, J. Van de Water, P.S.C. Leung, K. M. Humphries, L.I Szweda, Y. Nakanuma, T.E. Roche, R.L. Coppel, J-F. Bach, and M.E. Gershwin (2000) Comparative Immunoreactivity of Anti-trifluoroacetyl (TFA) Antibody and Anti-lipoic Acid Antibody in Primary Biliary Cirrhosis: Searching for a Mimic. J. of Autoimmun. 15, 51–60. [Article]

J. C. Baker, X. Yan, T. Peng, S. Kasten and T. E. Roche (2000) Marked Differences Between Two Isoforms of Human Pyruvate Dehydrogenase Kinase. J. Biol. Chem. 275, 15773-15781. [Article]

X. Gong, T. Peng, A. Yakhnin, M. Zolkiewski, J. Quinn, S. J. Yeaman, and T. E. Roche (2000) Specificity Determinants for the Pyruvate Dehydrogenase Component Reaction Mapped with Mutated and Prosthetic Group Modified Lipoyl Domains. J. Biol. Chem. 275,13645-13653. [Article]

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1990s

L. Dubel, A. Tanaka, P.S.C. Leung, J. Van De Water, R. Coppel, T.E. Roche, C. Johanet, Y. Motokawa, A. Ansari, M.E. Gershwin (1999) Autoepitope Mapping and Reactivity of Autoantibodies to the Dihydrolpoamide Dehydrogenase-Binding Protein (E3BP) and the Glycine Cleavage Proteins in Primary Biliary Cirrhosis. Hepatology 29, 1013-1018.

S. Shimoda, J. Van de Water, A. Ansari, M. Nakamura, H. Ishibashi, R.L. Coppel, J. Lake, E.B. Keeffe, T.E. Roche, and M.E. Gershwin (1998) Identification and Precursor Frequency Analysis of a Common T Cell Epitope Motif in Mitochondrial Autoantigens in Primary Biliary Cirrhosis. J. Clin. Invest. 102, 1831-1840. [Article]

D. Yang, X. Gong, A. Yakhnin, and T.E. Roche (1998) Requirements for the Adaptor Protein Role of Dihydrolipoyl Acetyltransferase in the Upregulated Function of the Pyruvate Dehydrogenase Kinase and Pyruvate Dehydrogenase Phosphatase. J. Biol. Chem. 273, 14130-14137. [Article]

D. Yang, J. Song, T. Wagenknecht, and T.E. Roche (1997) Assembly and Full Functionality of Recombinantly Expressed Dihydrolipoyl Acetyltransferase Component of the Human Pyruvate Dehydrogenase Complex. J. Biol. Chem. 272, 6361-6369. [Article]

G. Chen, L. Wang, S. Liu, C. Chang, and T.E. Roche (1996) Activated Function of the Pyruvate Dehydrogenase Phosphatase through Ca2+-facilitated Binding to the Inner Lipoyl Domain of the Dihydrolipoyl Acetyltransferase. J. Biol. Chem. 271, 28064-28070. [Article]

T.E. Roche and D.J. Cox (1996) Multifunctional 2-Oxo Acid Dehydrogenase Complexes in Channeling in Intermediary Metabolism (L. Agius and H.S.A. Sherratt, eds.), Portland Press Ltd., London, pp. 115-132.

T.E. Roche, J. Liu, S. Ravindran, J. Baker, and D. Wang (1996) Role of the E2 Core in the Dominant Mechanisms of Regulatory Control of Mammalian Pyruvate Dehydrogenase Complex, in a Keto Acid Dehydrogenase Complexes. (M.S. Patel, T.E. Roche and R.A. Harris, eds.) Burkhäuser Verlag Press, Basel, pp. 33-52.

M.S. Patel, T.E. Roche, R.A. Harris (Editors) (1996) Alpha-Keto Acid Dehydrogenase Complexes. Birkhaüser Press Basel, Preface XI.

S. Ravindran, G.A. Radke, and T.E. Roche (1996) Lipoyl Domain-Based Mechanism for Integrated Feedback Control of Pyruvate Dehydrogenase Complex by Enhancement of Pyruvate Dehydrogenase Kinase Activity. J. Biol. Chem. 271, 653-662. [Article]

D. Eswaran, M.S. Ali, B.C. Shenoy, L.G. Korotchkina, T.E. Roche, and M.S. Patel (1995) Arginine-239 in the Beta Subunit is at or near the Active Site of Pyruvate Dehydrogenase. Biochem. Biophys. Acta 1252, 203-208.

J. Van de Water, A. Ansari, S. Liu, T.E. Roche, T. Prinville, R. Coppel. N.N.L. Kotzin, S.M. Krams, S. Munoz, and M.E. Gershwin (1995) Heterogeneity of Autoreactive T Cell Clones Specific for the E2 Component of the Pyruvate Dehydrogenase Complex in Primary Biliary Cirrhosis. J. Exp. Med. 181, 723-733.

M. S. Ali, B. C. Shenoy, D. Eswaran, L. A. Andersson, T. E. Roche and M. S. Patel (1995) Identification of the tryptophan residue in the thiamin pyrophosphate binding site of bovine pyruvate dehydrogenase. J. Biol. Chem. 270, 4570-4574. [Article]

S. Liu, J.C. Baker, P.C. Andrews, and T. E. Roche (1995) Recombinant Expression and Evaluation of the Lipoyl Domains of the Dihydrolipoyl Acetyltransferase Component of Human Pyruvate Dehydrogenase Complex. Arch. Biochem. Biophys. 316, 926-940. [Article]

S. Liu, J.C. Baker, and T.E. Roche (1995) Binding of the Pyruvate Dehydrogenase Kinase to Recombinant Constructs Containing the Inner Lipoyl Domain of the Dihydrolipoyl Acetyltransferase Component. J. Biol. Chem. 270, 793-800. [Article]

M. S. Ali, T. E. Roche and M. S. Patel (1993) Identification of the essential cysteine residue in the active site of bovine pyruvate dehydrogenase. J. Biol. Chem. 268, 22353-22356.

K. Ono, G.A. Radke, T.E. Roche, and M. Rahmatullah (1993) Partial Activation of the Pyruvate Dehydrogenase Kinase by the Lipoyl Domain Region of E2 and Interchange of the Kinase Between Lipoyl Domain Regions. J. Biol. Chem. 268, 26135-26143.

T.E. Roche, S.L. Powers-Greenwood, W. Shi, W. Zhang, S.Z. Ren, E.D. Roche, D.J. Cox, and C.M. Sorensen (1993) Sizing of the Bovine Pyruvate Dehydrogenase Complex and the Dihydrolipoyl Transacetylase Core by Quasielastic Light Scattering. Biochemistry 32, 5629-5637.

G.A. Radke, K. Ono, S. Ravindran, and T.E. Roche (1993) Critical Role of a Lipoyl Cofactor of the Dihydrolipoyl Acetyltransferase in the Binding and Enhanced Function of the Pyruvate Dehydrogenase Kinase. Biochem. Biophys. Res. Comm. 190, 982-991.

L. Li, G.A. Radke, K. Ono, and T.E. Roche (1992) Additional Binding Sites for the Pyruvate Dehydrogenase Kinase but not for Protein X in the Assembled Core of the Mammalian Pyruvate Dehydrogenase Complex. Binding Region for the Kinase. Arch. Biochem. Biophys. 296, 497-504.

T. Wagenknecht, R. Grassucci, G.A. Radke and T.E. Roche (1991) Cryoelectron Microscopy of Mammalian Pyruvate Dehydrogenase Complex. J. Biol. Chemistry 266, 24650-24656.

I. Iwayana, P.S.C. Leung, R.L. Coppel, T.E. Roche, M.S. Patel, Y.M. Zushima, R. Dickson, and M.E. Gershwin (1991) Specific Reactivity of Recombinant Human PDC-E1a in Primary Biliary Cirrhosis. J. of Autoimmun. 4, 769-778.

T.E. Roche, M. Rahmatullah, C.L. Chang, G.A. Radke, S. Powers-Greenwood, S. Gopalakrishnan, and L. Li (1991) Subdomain Roles of the Lipoyl-bearing Components of Mammalian Pyruvate Dehydrogenase Complex, in Biochemistry and Physiology of Thiamine Diphosphate Enzymes (H. Bisswanger and H. Ulrich, eds.) VCH Publishers, Weinheim, Germany, pp. 164-169.

J. Van de Water, A.A. Ansari, C.D. Surh, R. Coppel, T.E. Roche, H. Bonkovsky, M. Kaplan, M.E. Gershwin (1991) Evidence for the Targeting by 2-0xo-dehydrogenase enzymes in the T Cell Response of Primary Biliary Cirrhosis. J. Immunology 146, 89-94.

M.S. Patel and T.E. Roche (1990) Molecular Biology and Biochemistry of Pyruvate Dehydrogenase Complexes FASEB J. 4, 3224-3233.

M. Rahmatullah, G.A. Radke, P.C. Andrews, T.E. Roche (1990) Changes in the Core of the Mammalian-Pyruvate Dehydrogenase Complex upon Selective Removal of the Lipoyl Domain from the Transacetylase Component But Not from the Protein X Component. J. Biol. Chem. 265, 14512-14517.

D.R. Fregeau, T.E. Roche, P.A. Davis, R. Coppel, M.E. Gershwin (1990) Primary Biliary Cirrhosis: Inhibition of Pyruvate Dehydrogenase Complex Activity by Autoantibodies Specific for E1a, A Non-Lipoic Binding Mitochondrial Enzyme. J. Immunol. 144 1671-1676.

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1980s

T.E. Roche, M. Rahmatullah, L. Li, G.A. Radke, C.L. Chang, S.L. Powers-Greenwood (1989) The Lipoyl-Containing Components of Mammalian Pyruvate Dehydrogenase Complex: Roles in Modulating and Anchoring the PDH kinase and PDH Phosphatase. Ann. N.Y. Acad. Sci. 573, 168-174.

T.E. Roche, M. Rahmatullah, S.L. Powers-Greenwood, G.A. Radke, S. Gopalakrishnan, and C.L. Chang (1989) The Lipoyl-Containing Components of the Mammalian Pyruvate Dehydrogenase Complex: Structural Comparison and Subdomain Roles. Ann. N.Y. Acad. Sci. 573, 66-75.

T.E. Roche (1989) Tribute to Lester J. Reed, Ann. N.Y. Acad. Sci. 573, XV-XVIII.

T.E. Roche and M.S. Patel (Editors) (1989) a-Keto Acid Dehydrogenase Complexes: Organization, Regulation, and Biomedical Ramifications. Ann. N.Y. Acad. Sci. Vol. 573.

C.D. Surh, T.E. Roche, D.J. Danner, A. Ahmed, R.L. Coppel, R. Dickson, M.E. Gershwin (1989) Antimitochondrial Autoantibodies in Primary Biliary Cirrhosis Recognize a Cross-reactive Epitope on Protein X. Hepatology 10, 127-133.

S. Gopalakrishnan, M. Rahmatullah, G.A. Radke, S.L. Powers-Greenwood, and T.E. Roche (1989) Role of Protein X in the Function of Mammalian Pyruvate Dehydrogenase Complex. Biochem. Biophys. Res. Commun., 160, 715-721.

S.L. Powers-Greenwood, M. Rahmatullah, G.A. Radke, and T.E. Roche (1989) Separation of Protein X from the Dihydrolipoyl Transacetylase Component of the Mammalian Pyruvate Dehydrogenase Complex and Role of Protein X. J. Biol. Chem., 264, 3655-3657.

M. Rahmatullah, S. Gopalakrishnan, P.C. Andrews, C.L. Chang, G.A. Radke, and T.E. Roche (1989) Subunit Associations in the Mammalian Pyruvate Dehydrogenase Complex: Structure and Role of Protein X and the Pyruvate Dehydrogenase Component Binding Domain of the Dihydrolipoyl Transacetylase Component. J. Biol. Chem. 264, 2221-2227.

M. Rahmatullah, S. Gopalakrishnan, G.A. Radke, and T.E. Roche (1989) Domain Structures of the Dehydrolipoyl Transacetylase and the Protein X Components of Mammalian Pyruvate Dehydrogenase Complex--Selective Cleavage by Protease Arg C. J. Biol. Chem. 264, 1245-1251.

M. Rahmatullah and T.E. Roche (1988) Component Requirements for NADH Inhibition and Spermine Stimulation of Pyruvate Dehydrogenaseb Phosphatase Activity. J. Biol. Chem. 263, 8106-8110.

T. E. Roche, M. Rahmatullah, J. M. Jilka and M. Kazemi (1988) Covalent Regulation of Pyruvate Dehydrogenase Kinase in Thiamin Pyrophosphate Chemistry Volume II (R. L. Schowen and A. Schellenberger eds.) CRC Press Uniscience Series pp. 93-101.

M. Rahmatullah and T. E. Roche (1987) The Catalytic Requirements for Reduction and Acetylation of Protein X and the Related Regulation of Various Forms of Resolved Pyruvate Dehydrogenase Kinase. J. Biol. Chem. 262, 10265-10271.

M. Rahmatullah, J. M. Jilka, G. A. Radke, and T. E. Roche (1986) Properties of the Pyruvate Dehydrogenase Kinase Bound to and Separated from the Dihydrolipoyl Transacetylase-Protein X Subcomplex and Evidence for Binding of the Kinase to Protein X. J. Biol. Chem. 261, 6515-6523.

J. M. Jilka, M. Rahmatullah, M. Kazemi and T. E. Roche (1986) Properties of a Newly Characterized Protein of the Bovine Kidney Pyruvate Dehydrogenase Complex. J. Biol. Chem. 261, 1858-1867.

M. Rahmatullah and T. E. Roche (1985) Modification of Bovine Kidney Pyruvate Dehydrogenase Kinase Activity by CoA Esters and Their Mechanism of Action. J. Biol. Chem. 260, 10146-10152.

M. Rahmatullah, T. E. Roche, J. M. Jilka and M. Kazemi (1985) Mechanism of Activation of Bovine Kidney Pyruvate Dehydrogenasea Kinase by Malonyl-CoA and Enzyme-catalyzed Decarboxylation of Malonyl-CoA. Eur. J. Biochem. 150, 181-87.

A. Dixit and T. E. Roche (1984) Spectrophotometric Assay of the Flavin-containing Monooxygenase and Changes in Its Activity in Female Mouse Liver with Nutritional and Diurnal Conditions. Arch. Biochem. Biophys. 233, 50-63.

D. R. Brandt and T. E. Roche (1983) Specificity of the Pyruvate Dehydrogenase Kinase for Pyruvate Dehydrogenase Component bound to the Surface of the Kidney Pyruvate Dehydrogenase Complex and Evidence for Intracore Migration of Pyruvate Dehydrogenase Component. Biochemistry 22, 2966-2971.

D. R. Brandt, T. E. Roche, and M. L. Pratt (1983) Heterogeneity of Binding Sites for the Pyruvate Dehydrogenase Component on the Dihydrolipoyl Transacetylase Component of Bovine Kidney Pyruvate Dehydrogenase Complex. Biochemistry 22, 2958-2965.

M. L. Pratt, J. C. Gold, and T. E. Roche (1982) Sepharose-insolubilization of the Dihydrolipoyl Transacetylase Core Component of the Pyruvate Dehydrogenase Complex: Preparation and Characterization. J. Bioch. Biophys. Meth. 6, 119-131.

M. L. Pratt, J. F. Maher, and T. E. Roche (1982) Purification of Bovine Kidney and Heart Pyruvate Dehydrogenase Phosphatase on Sepharose Derivatized with the Pyruvate Dehydrogenase Complex. Eur. J. Biochem. 125, 349-355.

T. P. O'Connor, T. E. Roche, and J. V. Paukstellis (1982) 13C Nuclear Magnetic Resonance Study of the Pyruvate Dehydrogenase Catalyzed Acetylation of Dihydrolipoamide. J. Biol. Chem. 257, 3110-3112.

T. E. Roche and V. B. Lawlis (1981) Structure and Regulation of a-Ketoglutarate Dehydrogenase of Bovine Kidney. Ann. N.Y. Acad. Sci. 378, 236-249.

V. B. Lawlis and T. E. Roche (1981) Inhibition of Bovine Kidney a-Ketoglutarate Dehydrogenase Complex by NADH in the Presence or Absence of Ca2+ and Effect of ADP on NADH Inhibition. Biochemistry 20, 2519-2524.

V. B. Lawlis and T. E. Roche (1981) Regulation of Bovine Kidney a-Ketoglutarate Dehydrogenase Complex by Ca2+ and Adenine Nucleotides; Effects on S0.5 for a-Ketoglutarate. Biochemistry 20, 2512-2518.

V. B. Lawlis and T. E. Roche (1980) Effect of Micromolar Ca2+ on NADH Inhibition of Bovine Kidney a-Ketoglutarate Dehydrogenase Complex and Possible Role of Ca2+ in Signal Amplification. Mol. Cell. Biochem. 32, 147-152.

R. L. Cate, T. E. Roche, and L. C. Davis (1980) Rapid Intersite Transfer of Acetyl Groups and Movement of Pyruvate Dehydrogenase Component in the Kidney Pyruvate Dehydrogenase Complex. J. Biol. Chem. 255, 7556-7562.

T. E. Roche, K. J. Kramer,and D. W. Dyer (1980) Regulation of Fat Body, Pyruvate Dehydrogenase Complex in the Tobacco Hornworm, Manduca sexta. Insect Biochem. 10, 577-582.

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1970s

M. L. Pratt, T. E. Roche, D. W. Dyer, and R. L. Cate (1979) Enhanced Dissociation of Pyruvate Dehydrogenase from the Pyruvate Dehydrogenase Complex following Phosphorylation and Regulatory Implications. Biochem. Biophys. Res. Commun. 91, 289-296.

L. C. Davis, R. L. Cate, and T. E. Roche (1979) A Simple, Reliable Rapid-mixing Apparatus for Continuous-flow Studies. Anal. Biochem. 97, 428-437.

M. L. Pratt and T. E. Roche (1979) Mechanism of Pyruvate Inhibition of Kidney Pyruvate Dehydrogenasea Kinase and Synergistic Inhibition by Pyruvate and ADP. J. Biol. Chem. 254, 7191-7196.

R. L. Cate and T. E. Roche (1979) Function and Regulation of Mammalian Pyruvate Dehydrogenase Complex: Acetylation, Interlipoyl Acetyl Transfer, and Migration of the Pyruvate Dehydrogenase Component. J. Biol. Chem. 254, 1659-1665.

S. J. Yeaman, E. T. Hutcheson, T. E. Roche, F. H. Pettit, J. R. Brown, L. J. Reed, D. C. Watson, and G. H. Dixon (1978) Sites of Phosphorylation on Pyruvate Dehydrogenase from Bovine Kidney and Heart. Biochemistry 17, 2364-2370.

R. L. Cate and T. E. Roche (1978) A Unifying Mechanism for Stimulation of Mammalian Pyruvate Dehydrogenase Kinase Activity by NADH, Dihydrolipoamide, Acetyl-CoA, or Pyruvate. J. Biol. Chem. 253, 496-503.

E. F. Bryne, T. P. O'Connor, T. E. Roche, and J. V. Paukstelis (1977) 13C Nuclear Magnetic Resonance spectra of Thiols: Lipoic Acid and Derivatives. J. Org. Chem. 42, 3941-3944.

T. E. Roche and R. L. Cate (1977) Purification of Porcine Liver Pyruvate Dehydrogenase Complex and Characterization of Its Catalytic and Regulatory Properties. Arch. Biochem. Biophys. 183, 664-677.

T. E. Roche and R. L. Cate (1976) Evidence for Lipoic Acid Mediated NADH and Acetyl-CoA Stimulation of Liver and Kidney Pyruvate Dehydrogenase Kinase. Biochem. Biophys. Res. Commun. 72, 1375-1383.

L. J. Reed, F. H. Pettit, T. E. Roche, J. W. Pelley and P. J. Butterworth (1976) Structure and Regulation of Mammalian Pyruvate Dehydrogenase Complex in "Metabolis Interconversion of Enzymes" (1975) (S. Shaltiel, ed.) Springer-Verlag, Berlin, pp. 121-124.

P. J. Butterworth, C. S. Tsai, M. H. Eley, T. E. Roche and L. J. Reed (1975) A Kinetic Study of Dihydrolipoyl Transacetylase from Bovine Kidney. J. Biol. Chem. 250, 1921-1925.

T. E. Roche and L. J. Reed (1974) Monovalent Cation Requirement for ADP Inhibition of Pyruvate Dehydrogenase Kinase. Biochem. Res. Commun. 59, 1341-1348.

L. J. Reed, F. H. Pettit, T. E. Roche and P. J. Butterworth, C. R. Barrera, C. S. Tsai (1974) Structure, Function, and Regulation of Mammalian Pyruvate Dehydrogenase Complex in "Third International Symposium on Metabolic Interconversion of Enzymes." (E. H. Fischer, E. G. Krebs, H. Neurath, E. R. Stadtman, eds.) Springer-Verlag Press, New York, pp. 99-106.

L. J. Reed, F. H. Pettit, T. E. Roche and P. J. Butterworth (1973) Regulation of the Mammalian Pyruvate Dehydrogenase Complex by Phosphorylation and Dephosphorylation in "Protein Phosphorylation in Control Mechanisms." (F. Huijing and E.Y.C. Lee, eds.) Academic Press, New York, pp. 83-97.

F. H. Pettit, T. E. Roche, L. J. Reed (1972) Function of Calcium Ions in Pyruvate Dehydrogenase Phosphatase Activity. Biochem. Biophys. Res. Commun. 49, 563-571.

T. E. Roche and L. J. Reed (1972) Function of the Nonidentical Subunits of Mammalian Pyruvate Dehydrogenase. Biochem. Biophys. Res. Commun. 48, 840-846.

F. Hucho, D. D. Randall, T. E. Roche, M. W. Burgett, J. W. Pelley, and L. J. Reed (1972) a-Keto Acid Dehydrogenase Complexes. XVII. Kinetic and Regulatory Properties of Pyruvate Dehydrogenase Kinase and Pyruvate Dehydrogenase Phosphatase from Bovine Kidney and Heart. Arch. Biochem. Biophys. 151, 328-340.

L. J. Reed, T. C. Linn, F. Hucho, G. Namihira, C. R. Barrera, T. E. Roche, J. W. Pelley and D. D. Randall (1972) Molecular Aspects of the Regulation of Mammalian Pyruvate Dehydrogenase Complex in "Second International Symposium on the Metabolic Interconversion of Enzymes." (O. Weiland, E. Hemlreich, and H. Holzer, eds.) Springer-Verlag, Berlin, pp. 281-291.

L. J. Reed, T. C. Linn, F. H. Pettit, R. M. Oliver, F. Hucho, J. W. Pelley, D. D. Randall and T. E. Roche (1972) Pyruvate Dehydrogenase Complex: Structure, Function, Regulation in "Energy Metabolism and the Regulation of Metabolic Processes in Mitochondria." (M. A. Mehlman and R. W. Hanson, eds.) Academic Press, New York, pp. 253-270.

T. E. Roche, B. A. McFadden and J. O. Williams (1971) Modification of the Active Site of Isocitrate Lyase from Pseudomonas indigofera. Arch. Biochem. Biophys. 147, 192-200.

J. O. Williams, T. E. Roche and B. A. McFadden (1971) Mechanism of Action of Isocitrate Lyase from Pseudomonas Indigofera. Biochemistry 10, 1384-1390.

T. E. Roche, J. O. Williams and B. A. McFadden (1970) Effect of pH and Buffer upon Km and Inhibition by Phosphoenolpyruvate of Isocitrate Lyase from Pseudomonas indigofera. Biochim. Biophys. Acta 206, 193-195.

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1960s

T. E. Roche and B. A. McFadden (1969) Active Site Modification of Isocitrate Lyase. Biochem. Biophys. Res. Commun. 37, 239-246.

B. A. McFadden, C. R. Rao, A. L. Cohen and T. E. Roche (1968) Isocitrate Lyase Pseudomonas indigofera. V. Subunits and Terminal Residues and the Relation to Catalytic Activity. Biochemistry 7, 3574-3582.

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