Department of Biochemistry & Molecular Biophysics
141 Chalmers Hall
Manhattan, KS 66506
785-532-6121
785-532-7278 fax
biochem@k-state.edu



Biotechnology Core Facility
206 Burt Hall
785-532-5956
785-532-6297 fax



Biomolecular NMR Facility
37 Chalmers Hall
785-532-2345

Ramaswamy Krishnamoorthi, Ph.D., Associate Professor
Advisor

Complete publications list

2000s | 1990s | 1980s

2010s

Takahashi, D., Dai, H., Hiromasa, Y., Krishnamoorthi, R., & Kanost, M.R. (2014) Self-association of an Insect b-1,3-Glucan Recognition Protein Upon Binding Laminarin Stimulates Prophenoloxidase Activation as Innate Immune Response. J. Biol. Chem. 289, 28399-28410. [Article]

Dai, H., Hiromasa, Y., Takahashi, D., VanderVelde, D., Fabrick, J., Kanost, M.R., & Krishnamoorthi, R. (2013) An Initial Event in the Insect Innate Immune Response: Structural and Biological Studies of Interactions between β ‑ 1,3-Glucan and the N ‑ Terminal Domain of β ‑ 1,3-Glucan Recognition Protein, Biochemistry 52, 161-170. [Article]

Karote, D., Walker, B., Dai, H., Krishnamoorthi, R., Voo, J., & Rajagopalan, S. (2013) Chemical warfare agent simulants in Gamble's fluid: Is the fluid toxic? Can it be made safer by inclusion of solid nanocrystalline metal oxides? Journal of Chemistry, DOI:10.1155/2013/641620. [Article]

2000s

Pappan, K., Zheng, L., Krishnamoorthi, R., and Wang, X. (2004) Evidence for and characterization of Ca2+ binding to the catalytic region of Arabidopsisthaliana phospholipase Dβ, J. Biol. Chem. 279:47833-47839. [Article]

Shan, J., Baguinon, M., Zheng, L., and Krishnamoorthi, R. (2003) Expression, refolding, and activation of the catalyltic domain of human blood coagulation factor XII, Protein Express. Purif. 27:143-149. [Article]

Cai, M., Gong, Y., Wen, L., and Krishnamoorthi, R. (2002) Correlation of binding-loop internal dynamics with stability and function in potato I inhibitor family: relative contributions of Arg50 and Arg52 in Cucurbita maxima trypsin inhibitor-V as studied by site-directed mutagenesis and NMR spectroscopy, Biochemistry 41: 9572-9579. [Article]

Zheng, L., Li, M., Shan, J., Krishnamoorthi, R., and Shen, B. (2002) Distinct roles of two Mg2+ binding sites in regulation of murine flap endonuclease-1 activities, Biochemistry 41: 10323-10331. [Article]

Zheng, L., Shan, J., Krishnamoorthi, R., and Wang, X. (2002) Activation of plant phospholipase Db by phosphatidylinositol 4,5-bisphosphate: Characterization of binding site and mode of action, Biochemistry 41: 4546-4553. [Article]

Zavodszky, M., Chen, C.-W., Huang, J.-K., Zolkiewski, M., Wen, L., and Krishnamoorthi, R. (2001) Disulfide bond effects on protein stability: Designed variants of Cucurbita maxima trypsin inhibitor-V, Protein Sci.10: 149-160. [Article]

Zheng, L., Krishnamoorthi, R., Zolkiewski, M., and Wang, X. (2000) Distinct Ca2+-binding properties of novel C2 domains of plant phospholipase Da and b, J. Biol. Chem. 275: 19700-19706. [Abstract]

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1990s

Liu, J., Y. Gong, O. Prakash, L. Wen, I. Lee, J.-K. Huang, and R. Krishnamoorthi, NMR studies of internal dynamics of serine proteinase protein inhibitors: Binding region mobilities of intact and reactive-site hydrolyzed Cucurbita maxima trypsin inhibitor (CMTI)-III of the squash family and comparison with those of counterparts of CMTI-V of the potato I family, Protein Sci.7, 132-141 (1998). [Abstract]

Liu, J., O. Prakash, Y. Huang, L. Wen, J.J. Wen, J.-K. Huang, and R. Krishnamoorthi, Internal mobility of reactive-site hydrolyzed recombinant Cucurbita maxima trypsin inhibitor-V characterized by NMR spectroscopy: Evidence for differential stabilization of newly formed C- and N-termini, Biochemistry 38, 12503-12510 (1996). [Abstract]

Cai, M., H. Huang, O. Prakash, L. Wen., S.P. Dunkelbarger, J.-K. Huang, J. Liu, and R. Krishnamoorthi, Differential Modulation of Binding Loop Flexibility and Stability by Arg50 and Arg52 in Cucurbita maxima Trypsin Inhibitor-V Deduced by Trypsin-Catalyzed Hydrolysis and NMR Spectroscopy, Biochemistry 35, 4784-4794 (1996). [Abstract]

Liu, J., O. Prakash, M. Cai, Y. Gong, L. Wen, J.P. Wen, and R. Krishnamoorthi, Solution structure and backbone dynamics of recombinant Cucurbita maxima trypsin inhibitor-V determined by NMR spectroscopy, Biochemistry 35, 1516-1524 (1996). [Abstract]

Wen, L., I. Lee, G. J. Chen, J.-K. Huang, Y. X. Gong, and R. Krishnamoorthi, Changing the inhibitory specificity and function of Cucurbita maxima trypsin inhibitor-V by site-directed mutagenesis, Biochem. Biophys. Res. Commun. 207, 897-902 (1995). [Abstract]

Cai, M., Y. Gong, O. Prakash, and R. Krishnamoorthi, Reactive-site hydrolyzed Cucurbita maxima trypsin inhibitor-V. Function, thermodynamic stability, and NMR solution structure, Biochemistry 34, 12087-12094 (1995). [Abstract]

Cai, M., Y. Gong, J. L. -F. Kao, and R. Krishnamoorthi, Three-dimensional solution structure of Cucurbita maxima trypsin inhibitor-V determined by NMR spectroscopy, Biochemistry 34, 5201-5211 (1995). [Abstract]

Cai, M., Y. Huang, J. Liu, and R. Krishnamoorthi, Solution conformations of proline rings in proteins studied by NMR spectroscopy, J. Biomol. NMR 6, 123-128 (1995). [Abstract]

Cai, M., Y. Huang, O. Prakash, L. Wen, S. K. Han, and R. Krishnamoorthi, Solution conformations and dynamics of arginine side chains in a protein deduced from three-bond 15N-1H coupling constants. J. Magn. Reson. Series B 108, 189-191 (1995). [Abstract]

Cai, M., J. Liu, Y. Gong, and R. Krishnamoorthi, A practical method for stereospecific assignments of g- and d-methylene hydrogens via estimation of vicinal 1H-1H coupling constants, J. Magn. Reson. Series B107, 172-178 (1995). [Abstract]

Cai, M., Y. Gong, and R. Krishnamoorthi, Identification of hydrogen-bonded lysine and arginine residues in a protein by means of X4-4 torsional-angle determination, J. Magn. Reson., Ser. B 106, 297-299 (1995). [Abstract]

Vyas, K., Rajarathnam, K., Yu, L.P., Emerson, S.D., La Mar G.N., Krishnamoorthi, R., and Mizukami, H. 1H NMR investigation of the heme cavity of elephant (E7 Gln) met-cyano-myoglobin. Evidence for a B-helix phenylalanine interaction with bound ligand, J. Biol. Chem. 268, 14826-14835 (1993). [Abstract]

L. Wen, S.-S. Kim, T.T. Tinn, J.-K. Huang, R. Krishnamoorthi, Y.-X. Gong, & Y. N. Lwin, Chemical Synthesis, Molecular Cloning, Overexpression and Site-Directed Mutagenesis of the Gene Coding for Pumpkin (Cucurbita maxima) Trypsin Inhibitor V (CMTI-V), Protein Express. Purif. 4, 215-222 (1993). [Abstract]

R. Krishnamoorthi, S. Nemmers, & B. Tobias, Natural Abundance 15N NMR Assignments Delineate Structural Differences between Intact and Reactive-Site Hydrolyzed Cucurbita maxima Trypsin Inhibitor III, FEBS Lett. 304, 149-152 (1992). [Abstract]

R. Krishnamoorthi, C.S. Lin, & D. VanderVelde, Structural Consequences of the Natural Substitution, E9K, on Reactive-Site Hydrolyzed Cucurbita maxima Trypsin Inhibitor III (CMTI-III), as Studied by Two-Dimensional NMR, Biochemistry 31, 4965-4969 (1992). [Abstract]

R. Krishnamoorthi, C.S. Lin, Y.-X. Gong, D. VanderVelde, & K. Hahn, Hydrogen-1 NMR Studies of Cucurbita maxima Trypsin Inhibitors: Evidence for pH-Dependent Conformational Change and His25-Tyr27Interaction, Biochemistry 31, 905-910 (1992). [Abstract]

R. Krishnamoorthi, Y.-X. Gong, C.S. Lin, & D. VanderVelde, Two-Dimensional NMR Studies of Squash Family Inhibitors. Sequence-Specific Hydrogen-1 Assignments and Secondary Structure of Reactive-Site Hydrolyzed Cucurbita maxima Trypsin Inhibitor III, Biochemistry31, 898-904 (1992). [Abstract]

R. Krishnamoorthi, Y.-X. Gong, & M. Richardson, A New Protein-Inhibitor of Trypsin and Activated Hageman Factor from Pumpkin (Cucurbita maxima) Seeds, FEBS Lett. 273, 163-167 (1990). [Abstract]

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1980s

R. Krishnamoorthi, M.A. Cusanovich, T.E. Meyer & C.T. Przysiecki, Hydrogen-1 NMR studies of High-Potential Iron-Sulfur Protein from the Purple Phototrophic Bacterium, Rhodospirillum tenue, Eur. J. Biochem. 181, 81-85 (1989). [Abstract]

G. N. La Mar, M.J. Chatfield, D.H. Peyton, J.D. deRopp, W.S. Smith, R. Krishnamoorthi, J.D. Satterlee, and J.E. Erman, Solvent Isotope Effects on NMR Spectral Parameters in High-Spin Ferric Hemoproteins: an Indirect Probe for Distal Hydrogen Bonding, Biochim. Biophys. Acta 956, 267-276 (1988). [Abstract]

D. H. Peyton, R. Krishnamoorthi, G. N. La Mar, K. Gersonde, K. M. Smith, & D. W. Parish, Protein-Structural Heterogeneity in a Non-Allosteric Monomeric Insect Hemoglobin Monitored by Proton Magnetic Resonance Spectroscopy, Eur. J. Biochem. 168, 377-383 (1987). [Abstract]

J. L. Markley, T.-M. Chan, R. Krishnamoorthi, & E.L. Ulrich, Nuclear Magnetic Resonance Studies of Structure-Function Relationships in Iron-Sulfur Proteins, in Iron-Sulfur Protein Research (H. Matsubara et al., eds.), Jap. Sci. Soc. Press, Tokyo/Springer-Verlag, Berlin, pp. 167-181 (1987).

G. Ortiz-Polo, R. Krishnamoorthi, J.L. Markley, D. Live, D.G. Davis, & D. Cowburn, Natural Abundance Nitrogen-15 NMR Studies of Turkey Ovomucoid Third Domain: Assignment of Peptide Nitrogen-15 Resonances to the Residues at the Reactive Site Region via Proton-Detected Multiple quantum Coherence, J. Magn. Reson. 68, 303-310 (1986). [Abstract]

P. M. Green, C. J. Wurrey, R. Krishnamoorthi, & Y. Y. Yeh, Vibrational Spectra of Cyclopropyl Cyanide and Cyclopropyl Cyanide-a-d1, J. Raman Spectros. 17, 355-362 (1986). [Abstract]

R Krishnamoorthi, J.L. Markley, M.A. Cusanovich, & C.T. Przysiecki, Hydrogen-l NMR Investigation of Clostridium pasteurianum Rubredoxin. Previously Unobserved Signals, Biochemistry 25, 50-54 (1986). [Abstract]

R. Krishnamoorthi, J.L. Markley, M.A. Cusanovich, C.T. Przysiecki, & T.E. Meyer, Hydrogen-1 NMR Investigation of High-Potential Iron-Sulfur Proteins from Ectothiorhodospira halophila and Ectothiorhodospira vacuolata. A Comparative Study of Hyperfine-Shifted Resonances, Biochemistry 25, 60-67 (1986). [Abstract]

J. L. Markley, D. H. Croll, R. Krishnamoorthi, G. Ortiz-Polo, W. M. Westler, W. C. Bogard, Jr., & M. Laskowski, Jr., One- and Two-Dimensional NMR Spectral Analysis of the Consequences of Single Amino Acid Replacements in Proteins, J. Cellular Biochem. 30, 29l-309 (1986). [Abstract]

G.N. La Mar, H. Toi, & R. Krishnamoorthi, Proton NMR Investigation of the Rate and Mechanism of Heme Rotation in Sperm Whale Myoglobin: Evidence for Intramolecular Reorientation about a Heme Twofold Axis, J. Am. Chem. Soc. 106, 6395-640l (1984). [Abstract]

C.J. Wurrey, Y.Y. Yeh, R. Krishnamoorthi, R.J. Berry, J.E. DeWitt, & V.F. Kalasinsky, Vibrational Spectra and Conformations of (Iodomethyl) Cyclopropane and Epiiodohydrin, J. Phys. Chem. 88, 4059-4063 (1984). [Abstract]

R. Krishnamoorthi, G.N. La Mar, H. Mizukami, & A. Romero, A 1H NMR Comparison of the Met-Cyano Complexes of Elephant and Spermwhale Myoglobin. Assignment of Labile Proton Resonances in the Heme Cavity and Determination of the Distal Glutamine Orientation from Relaxation Data, J. Biol. Chem. 259, 8826-8831 (1984). [Abstract]

R. Krishnamoorthi, G.N. La Mar, H. Mizukami, & A. Romero, A Proton NMR Investigation of the Influence of Distal Glutamine on Structural and Dynamic Properties of Elephant Metmyoglobin, J. Biol. Chem. 259, 265-270 (1984). [Abstract]

R. Krishnamoorthi & G.N. La Mar, Identification of the Titrating Group in the Heme Cavity of Myoglobin-Evidence for the Heme Protein p-p Interaction, Eur. J. Biochem. l38, l35-l40 (1984). [Abstract]

G. N. La Mar, R. Krishnamoorthi, K. M. Smith, K. Gersonde, & H. Sick, Proton Nuclear Magnetic Resonance Investigation of the Conformation-Dependent Spin Equilibrium in Azide-Ligated Monomeric Insect Hemoglobins, Biochemistry 22, 6239-6246 (1983). [Abstract]

G.N. La Mar & R. Krishnamoorthi, Proton Magnetic Resonance Study of the Influence of Heme 2,4 Substituents on the Exchange Rates of Labile Protons in the Heme Pocket of Myoglobin, Biophys. J. 44, l77-l83 (1983). [Abstract]

T. Jue, R. Krishnamoorthi, & G.N. La Mar, Proton NMR Study of the Mechanism of the Heme-Apoprotein Reaction for Myoglobin, J. Am. Chem. Soc. l05, 570l-5703 (1983). [Abstract]

C. J. Wurrey, R. Krishnamoorthi, S. Pechsiri, & V. F. Kalasinsky, Vibrational Spectra and Conformations of (Bromomethyl) Cyclopropane and Epibromohydrin, J. Raman Spectros. 12, 95-101 (1982). [Abstract]

C. J. Wurrey, R. Krishnamoorthi, & A. B. Nease, Vibrational Spectra and Structure of Cyclopropyl Isothiocyanate, J. Raman Spectrosc. 9, 334-338 (1980). [Abstract]

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