Department of Biochemistry & Molecular Biophysics
141 Chalmers Hall
Manhattan, KS 66506
785-532-6121
785-532-7278 fax
biochem@k-state.edu



Biotechnology Core Facility
206 Burt Hall
785-532-5956
785-532-6297 fax



Biomolecular NMR Facility
37 Chalmers Hall
785-532-2345

Michael Kanost, Ph.D., University Distinguished Professor
Advisor

Complete publications list

1970s | 1980s | 1990s | 2000s | 2010s

1970s

Capinera, J.L. and Kanost, M.R. (1979) Susceptibility of the zebra caterpillar to Autographa californica nuclear polyhedrosis virus. J. Econ. Entomol. 72: 570-572.


1980s

Dunn, P.E., Dai, W., Kanost, M.R. and Geng, C. (1985) Soluble peptidoglycan fragments stimulate antibacterial protein synthesis by fat body from larvae of Manduca sexta. Dev. Comp. Immunol. 9:559-568.

Wyatt, G.R., Kanost, M.R., Locke, J. and Walker, V.K. (1986) Juvenile hormone regulated locust vitellogenin genes: lack of expression after transfer into Drosophila. Arch. Insect Biochem. Physiol. Supplement 1:35-46.

Kanost, M.R., McDonald, H.L., Locke, J., Bradfield, J.Y. and Wyatt, G.R. (1987) Cloning and expression of the gene for apolipophorin-III from Locusta migratoria. In "Molecular Entomology" UCLA Symposia on Molecular and Cellular Biology, New Series, Volume 49. J. Law, (ed.) Alan R. Liss, Inc., New York. pp. 275-284.

Dunn, P.E., Kanost, M.R., and Drake, D.R. (1987) Increase in serum lysozyme following injection of bacteria into larvae of Manduca sexta. In "Molecular Entomology" UCLA Symposia on Molecular and Cellular Biology, New Series, Volume 49. J. Law (ed.) Alan R. Liss, Inc., New York. pp. 381-390.

Holden, H.M., Kanost, M.R., Law, J.H., Rayment, I. and Wells, M.A. (1988) Crystallization and preliminary analysis of crystals of apolipophorin-III isolated from Locusta migratoria. J. Biol. Chem. 263:3960-3962.

Kanost, M.R., Dai, W. and Dunn, P.E. (1988) Peptidoglycan fragments elicit antibacterial protein synthesis in larvae of Manduca sexta. Arch. Insect Biochem. Physiol. 8:147-164.

Kanost, M.R., Boguski, M.S., Freeman, M., Gordon, J.I., Wyatt, G.R. and Wells, M.A. (1988) Primary structure of apolipophorin-III from the migratory locust, Locusta migratoria: potential amphipathic structures and molecular evolution of an insect apolipoprotein. J. Biol. Chem. 263:10568-10573.

Kanost, M.R., Bradfield, J.Y., Cook, K.E., Locke, J., Wells, M.A. and Wyatt, G.R. (1988) Gene structure, cDNA sequence and developmental regulation of a low molecular weight hemolymph protein from Locusta migratoria. Arch. Insect Biochem. Physiol. 8:203-217.

Kanost, M.R., Prasad, S.V. and Wells, M.A. (1989) Primary structure of a member of the serpin superfamily of proteinase inhibitors from an insect, Manduca sexta. J. Biol. Chem. 264:965-972.

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1990s

Kanost, M.R. (1990) Isolation and characterization of four serine proteinase inhibitors (serpins) from hemolymph of Manduca sexta. Insect Biochem. 20:141-147.

Ladendorff, N.E. and Kanost, M.R. (1990) Isolation and characterization of bacteria-induced protein P4 from hemolymph of Manduca sexta. Arch. Insect Biochem. Physiol. 15:33-41.

Strobel, L.M., Kanost, M.R., Ziegler, R. and Wells, M.A. (1990) Adipokinetic hormone causes formation of a low density lipophorin in the house cricket, Acheta domestica. Insect Biochem. 20:859-863.

Kanost, M.R., Kawooya, J.K., Law, J.H., Ryan, R.O., Van Heusden, M.C. and Ziegler, R. (1990) Insect Haemolymph Proteins. Adv. Insect Physiol. 22:299-396.

Kanost, M.R. (1990) Serine protease inhibitors from the serpin gene family in Manduca sexta and Drosophila melanogaster. In "Molecular Insect Science," H.H. Hagedorn, J.G. Hildebrand, M.G. Kidwell and J.H. Law (eds.), Plenum, New York, pp. 139-146.

Van der Horst, D.J., Van Doorn, J.M., Voshol, H., Kanost, M.R., Ziegler, R. and Beenakkers, Ad M. Th. (1991) Different isoforms of an apoprotein (apolipophorin III) associate with lipoproteins in Locusta migratoria. Eur. J. Biochem. 196:509-517.

Breiter, D.R., Kanost, M.R., Benning, N.M., Law, J.H., Wells, M.A., Rayment, I. and Holden, H.M. (1991) Molecular structure of an apolipoprotein determined at 2.5 Å resolution. Biochem.30:603-608.

Ladendorff, N.E. and Kanost, M.R. (1991) Bacteria-induced protein P4 (hemolin) from Manduca sexta: a member of the immunoglobulin superfamily which can inhibit hemocyte aggregation. Arch. Insect Biochem. Physiol. 18:285-300.

Hanneman, E. and Kanost, M.R. (1992) Differential alaserpin expression during development of the antennae in the tobacco hawkmoth, Manduca sexta. Arch. Insect Biochem. Physiol. 19:39-52.

Wyatt, G.R., Kanost, M.R., Chin, B.C., Cook, K.E., Kawosoe, B.M. and Zhang, J. (1992) Juvenile hormone analog and injection effects on locust hemolymph protein synthesis. Arch. Insect Biochem. Physiol. 20:167-180.

Lin, Y., Hamblin, M.T., Edwards, M.J., Barillas-Mury, C., Kanost, M.R., Knipple, D.C., Wolfner, M.F. and Hagedorn, H.H. (1993) Structure, expression, and hormonal control of genes from the mosquito, Aedes aegypti, which encode proteins similar to the vitelline membrane proteins of Drosophila melanogaster. Dev. Biol. 155:558-568.

Beckage, N.E. and Kanost, M.R. (1993) Effects of parasitism by the braconid wasp Cotesia congregata on host hemolymph proteins of the tobacco hornworm, Manduca sexta.Insect Biochem. Molec. Biol. 23:643-653. 

Sugumaran, M. and Kanost, M.R. (1993) Regulation of insect hemolymph phenoloxidases. In "Parasites and Pathogens of Insects" (N. Beckage, S.N. Thompson, B.A. Federici, eds.) Academic Press. pp. 317-342.

Jiang, H., Wang, Y. and Kanost, M.R. (1994) Mutually exclusive exon use and reactive center diversity in insect serpins. J. Biol. Chem.269:55-58.

Kanost, M.R., Zepp, M,K., Ladendorff, N.E. and Andersson, L.A. (1994) Isolation and characterization of a hemocyte aggregation inhibitor from hemolymph of Manduca sexta larvae. Arch. Insect Biochem. Physiol. 27:123-136.

Smith, A.F., Owen, L., Strobel, L., Chen, H., Kanost, M., Hanneman, E. and Wells, M.A. (1994) Insect apolipophorins-III share a common function, but minimal amino acid sequence identity. Are amphipathic α-helices the common structural motif?J. Lipid Res. 35: 1976-1984.

Willott, E., Trenczek, T., Thrower, L. and Kanost, M.R. (1994) Immunochemical identification of insect hemocyte populations: Monoclonal antibodies which distinguish four major hemocyte types in Manduca sexta. Eur. J. Cell Biol. 65:417-423.

Kanost, M.R., Sparks, K.A. and Wells, M.A. (1995) Isolation and characterization of apolipophorin-III from the giant water bug, Lethocerus medius. Insect Biochem. Molec. Biol.25:759-764.

Kanost, M.R., Prasad, S.V., Huang, Y. and Willott, E. (1995) Regulation of serpin gene-1 inManduca sexta. Insect Biochem. Molec. Biol. 25:285-291.

Thomas, J.C., Adams, D.G., Keppenne, V.D., Wasmann, C.C., Brown, J.K., Kanost, M.R. and Bohnert, H.J. (1995) Manduca sexta encoded protease inhibitors expressed in Nicotiana tabacum provide protection against insects. Plant Physiol. Biochem. 33:611-614.

Wang, Y., Willott, E. and Kanost, M.R. (1995) Organization and expression of the hemolin gene, a member of the immunoglobulin superfamily in an insect, Manduca sexta. Insect Mol. Biol. 4:113-123.

Jiang, H.B., Mulnix, A.B. and Kanost, M.R. (1995) Expression and characterization of recombinantManduca sexta serpin-1B and site-directed mutants that change its inhibitory selectivity. Insect Biochem. Molec. Biol. 25:1093-1100.

Thomas, J.C., Adams, D.G., Keppenne, V.D., Wasmann, C.C., Brown, J.K., Kanost, M.R. and Bohnert, H.J. (1995) Protease inhibitors of Manduca sexta expressed in transgenic cotton. Plant Cell Reports. 14:758-762.

Willott, E., Lowenberger, C., Christensen, B.M. and Kanost, M.R. (1995) Monoclonal antibodies against Manduca sexta hemocytes bind Aedes aegypti hemocytes: characterization of six monoclonal antibodies that bind hemocytes from both species. Dev. Comp. Immunol. 19:451-461.

Jiang, H., Wang, Y. and Kanost, M.R. (1996) Primary structure of ribosomal proteins S3 and S7 fromManduca sexta. Insect Molec. Biol. 5:31-38.

Zhao, L. and Kanost, M.R. (1996) In search of a function for hemolin, a hemolymph protein from the immunoglobulin superfamily. J. Insect Physiol. 42: 73-79.

Jiang H., Wang, Y., Huang, Y., Mulnix, A.B., Kadel, J., Cole, K. and Kanost, M.R. (1996) Organization of serpin gene-1 from Manduca sexta: evolution of a family of alternate exons encoding the reactive site loop. J. Biol. Chem. 271: 28,017-28,023

Kanost, M.R. and Zhao, L. (1996) Insect hemolymph proteins from the Ig superfamily. Advances in Comparative and Environmental Physiology, 23:185-197.

Kanost, M.R. and Jiang, H. (1996) Proteinase inhibitors in invertebrate immunity. In "New Directions in Invertebrate Immunology" (K. Söderhäll, S. Iwanaga, G.R. Vasta, eds) SOS Publications, Fair Haven, NJ. pp. 155-174.

Jiang, H. and Kanost, M.R. (1997) Characterization and functional analysis of twelve naturally occurring reactive site variants of serpin-1 from Manduca sexta. J. Biol. Chem. 272:1082-1087.

Jiang, H., Wang, Y., Korochkina, S.E., Beneš, H. and Kanost, M.R. (1997) Molecular cloning of cDNAs for two pro-phenol oxidase subunits from the malaria vector, Anopheles gambiae. Insect Biochem. Molec. Biol. 27: 693-699.

Jiang, H., Wang, Y., Ma, C. and Kanost, M.R. (1997) Subunit composition of pro-phenol oxidase from Manduca sexta: molecular cloning of subunit proPO-p1. Insect Biochem. Molec. Biol. 27: 835-850.

Reeck, G., Kramer, K., Baker, J., Kanost, M., Fabrick, J. and Behnke, C. (1997) Proteinase inhibitors and resistance of transgenic plants to insects. In: "Advances in Insect Control, The Role of Transgenic Plants," (N. Carozzi and M. Koziel, eds.) Taylor and Francis, Brostol, PA, pp. 157-183.

Gillespie, J.P., Kanost, M.R. and Trenczek, T. (1997) Biological Mediators of Insect Immunity. Ann. Rev. Entomol. 42: 611-643.

Kanost, M.R. and Jiang, H. (1997) Serpins from an insect, Manduca sexta. In "Chemistry and Biology of Serpins" (F.C. Church, D.D.Cunningham, D. Ginsburg, M. Hoffman, S.R. Stone and D.M. Tollefsen, eds) Plenum, New York, pp. 155-161.

Trenczek, T. and Kanost, M.R. (1997) Insect Haemocytes: multifunctional cells in defense mechanisms. Sbornik (Czech Republic Academy of Science) 14:5-18.

Jiang, H., Wang, Y. and Kanost, M.R. (1998) Pro-phenol oxidase activating proteinase from an insect, Manduca sexta: a bacteria-inducible protein similar to Drosophila easter. Proc. Natl. Acad. Sci. USA. 95: 12220-12225.

Faye, I. and Kanost, M.R. (1998) Function and regulation of hemolin. In "Molecular Mechanisms of Immune Responses in Insects" (P.T. Brey and D. Hultmark, eds) Chapman and Hall, New York, pp. 173-188.

Trenczek, T., Willott, E. and Kanost, M.R. (1998) Preparation of monoclonal antibodies to insect hemocytes. In "Techniques in Insect Immunology," (A. Wiesner, G.B. Dunphy, V. J. Maramaras, I. Morishima, M. Sugumaran, M. Yamakawa, eds) SOS Publications, Fair Haven, NJ, pp. 21-32.

Jiang, H., Wang, Y. and Kanost, M.R. (1999) Four serine proteinases expressed in Manduca sexta hemocytes. Insect Mol. Biol. 8: 39-53.

Li, J., Wang, Z., Canagarajah, B., Jiang, H., Kanost, M.R. and Goldsmith, E.J. (1999) The structure of active serpin K from Manduca sexta and a model for serpin-protease complex formation. Structure. 7:103-109.

Zang, X., Yazdanbakhshy, M., Jiang, H., Kanost, M.R. and Maizels, R.M. (1999) A novel serpin expressed by blood-borne microfilariae of the parasitic nematode Brugia malayi inhibits human neutrophil serine proteinases. Blood, 94: 1418-1428.

Yu, X.-Q., Gan, H. and Kanost, M.R. (1999) Immulectin, an inducible C-type lectin from an insect, Manduca sexta, stimulates activation of plasma prophenol oxidase. Insect Biochem. Molec. Biol. 29:585-597.

Wang, Y., Jiang, H. Kanost, M.R. (1999) Biological activity of Manduca sexta paralytic and plasmatocyte spreading peptide and primary structure of its hemolymph precursor. Insect Biochem. Molec. Biol. 29: 1075-1086.

Yu, X.-Q., Prakash, O. and Kanost, M.R. (1999) Structure of a paralytic peptide from an insect,Manduca sexta. J. Peptide Res. 54: 256-261.

Yu, X.-Q. and Kanost, M.R. (1999) Developmental expression of Manduca sexta hemolin. Arch. Insect. Biochem. Physiol. 42: 198-212.

Reeck, G., Oppert, B., Denton, M, Kanost, M, Baker, J., Kramer K. (1999) Insect Proteinases. In “Proteases: New Perspectives” (V. Turk, ed.) Birkhauser, Basel, pp. 125-148.

Kanost, M.R. (1999) Serine proteinase inhibitors in arthropod immunity. Dev. Comp. Immunol. 23:291-301.

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2000s

Fröbius, A., Kanost, M.R., Götz, P. and Vilcinskas, A. (2000) Isolation and characterization of novel inducible serine protease inhibitors from larval hemolymph of the greater wax mothGalleria mellonella. Eur. J. Biochem. 267: 2046-2053.

Ma, C. and Kanost, M.R. (2000) A β,1-3-glucan-recognition protein from an insect, Manduca sexta, agglutinates microorganisms and activates the phenoloxidase cascade. J. Biol. Chem. 275: 7505-7514.

Yu, X.-Q. And Kanost, M.R. (2000) Immulectin-2, a lipopolysaccharide-specific lectin from an insect, Manduca sexta, is induced in response to Gram-negative bacteria. J. Biol. Chem.275:37373-37381.

Wiegand, C., Levin, D., Gillespie, J.P., Willott, W., Kanost, M.R. and Trenczek, T. (2000) Monoclonal antibody MS13 identifies a plasmatocyte membrane protein and inhibits encapsulation and spreading reactions of Manduca sexta hemocytes. Arch. Insect Biochem. Physiol. 45: 95-108.

Jiang, H. and Kanost, M.R. (2000) The clip-domain family of serine proteinases in arthropods. Insect Biochem. Molec. Biol. 30: 95-105.

Kramer, K.J. Kanost, M.R. Hopkins, T.L., Jiang, H., Xu, R., Zhu, Y.C., Kerwin, J.L. and Turecek, F. (2001) Oxidative conjugation of catechols in insect skeletal systems. Tetrahedron. 57: 385-392.

Nardi, J.B., Gao, C. and Kanost, M.R. (2001) The extracellular matrix protein lacunin is dynamically expressed by a subset of the hemocytes involved in basal lamina morphogenesis. J. Insect Physiol. 47:997-1006

Gan, H., Wang, Y., Jiang, H., Mita, K. and Kanost, M.R. (2001) A bacteria-induced, intracellular serpin in granular hemocytes of Manduca sexta. Insect Biochem. Molec. Biol. 31: 887-898.

Ye, S., Cech, A.L., Belmares, R., Bergstrom, R.C., Tong, Y., Corey, D.R., Kanost, M.R. and Goldsmith, E.J. (2001) The structure of a Michaelis serpin-protease complex. Nature Struct. Biol. 8:979-983.

Wang, Y., Jiang, H. and Kanost, M.R. (2001) Expression and purification of Manduca sextaprophenoloxidase-activating proteinase precursor (proPAP) from baculovirus-infected insect cells. Protein Express. Purif. 23: 328-337.

Kanost, M.R., Jiang, H., Wang, Y., Yu, X.-Q., Ma, C.C. and Zhu, Y. (2001) Hemolymph proteinases in immune responses of Manduca sexta.Adv. Exp. Med. Biol. 484: 319-28.

Yu, X-Q. and Kanost, M.R. (2001) A family of C-type lectins in Manduca sexta. Adv. Exp. Med Biol. 484: 191-194.

Jiang, H., Wang, Y. and Kanost, M.R. (2001) Proteolytic activation of prophenoloxidase in an insect, Manduca sexta. Adv. Exp. Med. Biol. 484: 313-317.

Ma, C. and Kanost, MR (2001). A β-1,3-glucan-binding protein from Manduca sexta. Adv. Exp. Med. Biol. 484: 309-12.

Wang, Y., Jiang, H. and Kanost, M.R. (2001) Paralytic and plasmatocyte spreading peptide from Manduca sexta.Adv. Exp. Med. Biol. 484: 373-375.

Yu, X.-Q., Prakash, O., Kanost, M.R. (2001) Insect ENF family peptides with diverse biological activities display similar core structures in solution. Medicinal Chem. Res. 10: 493-501.

Yu,X.-Q., Zhu, Y.-F., Ma, C, Fabrick, J.A. and Kanost, M.R. (2002) Pattern recognition proteins inManduca sexta plasma. Insect Biochem. Molec.Biol. 32: 1287-1293.

Yu, X.-Q. and Kanost, M.R. (2002) Binding of hemolin to microbial lipopolysaccharide and lipoteichoic acid: an immunoglobulin superfamily member from insects as a pattern recognition receptor. Eur. J. Biochem. 269: 1827-1834.

Zhu, Y.-C., Specht, C., Dittmer, N., Muthukrisnan, S., Kanost, M.R. and Kramer, K.J. (2002) Sequence of a cDNA and expression of the gene encoding a putative epidermal chitin synthase of Manduca sexta. Insect Biochem. Molec. Biol. 32: 1497-1506.

Swist, S.L., Wilkerson, M.J., Wyatt, C.R., Broce, A.B. and Kanost, M.R. (2002) Modulation of bovine lymphocyte responses by salivary gland extract of the stable fly Stomoxys calcitrans (Diptera: Muscidae). J. Med. Entomol. 36: 900-907.

Yu, X.-Q., Jiang, H., Wang, Y. and Kanost, M.R. (2003) Nonproteolytic serine proteinase homologs involved in phenoloxidase activation in the tobacco hornworm, Manduca sexta. Insect Biochem. Molec. Biol. 33: 197-208.

Jiang, H., Wang, Y., Yu, X.-Q. and Kanost, M.R. (2003) Prophenoloxidase-activating proteinase-2 (PAP-2) from hemolymph of Manduca sexta: a bacteria-inducible serine proteinase containing two clip domains. J. Biol. Chem. 278: 3552-3561.

Ross, J., Jiang, H., Kanost, M.R. and Wang, Y. (2003) Serine proteinases and their homologs from the Drosophila melanogaster genome: an initial analysis of sequence conservation and phylogenetic relationship. Gene 304:117-131.

Yu, X.-Q. and Kanost, M.R. (2003) Manduca sexta lipopolysaccharide-specific immulectin-2 protects larvae from bacterial infection. Dev. Comp. Immunol. 27: 189-196.

Zhu, Y., Johnson, T., Myers, A. and Kanost, M.R. (2003) Identification by subtractive suppression hybridization of bacteria-induced genes expressed in Manduca sexta fat body. Insect Biochem. Molec. Biol. 33, 541-559.

Suderman, R.J., Andersen S.O., Hopkins, T.L., Kanost, M.R. and Kramer, K.J. (2003) Characterization and cDNA cloning of three major proteins from pharate pupal cuticle of Manduca sexta. Insect Biochem. Mol. Biol. 33: 331-343.

Fabrick, J.A., Baker, J.E. and Kanost, M.R. (2003) cDNA cloning, purification, properties, and functions of a β-1,3-glucan recognition protein from a pyralid moth, Plodia interpunctella. Insect Biochem. Molec. Biol. 33: 579-594.

Kanost, M.R. (2003) Hemolymph. In Encyclopedia of Insects (V.H. Resh and R. Carde, eds) Academic Press. New York, pp. 505-508.

Jiang, H., Wang, Y., Yu, X.-Q., Zhu, Y., and Kanost, M.R. (2003) Prophenoloxidase-activating proteinase-3 (PAP-3) from Manduca sexta hemolymph: a clip-domain serine proteinase regulated by serpin-1J and serine proteinase homologs. Insect Biochem. Molec. Biol. 33: 1049-1060.

Shen, Z., Denton, M., Mutti, N. Pappan, K., Kanost, M.R., Reese, J.C. and Reeck, G.R. (2003) Polygalacturonase from Sitophilus oryzae: Possible horizontal transfer of a pectinase gene from fungi to weevils. Journal of Insect Science, 3:24.

Nardi, J.B., Pilas, B., Ujhelyi, E. Garsha, K., and Kanost, M.R. (2003) Hematopoietic organs of Manduca sexta and hemocyte lineages. Dev. Genes Evol. 213: 477-491.

Zhu, Y., Wang, Y., Gorman, M.J.. Jiang, H., and Kanost, M. R. (2003) Manduca sexta serpin-3 regulates prophenoloxidase activation in response to infection by inhibiting prophenoloxidase-activating proteinases. J. Biol. Chem. 278: 46,556-46,564. 

Gorman, M.J., Kankanala, P. And Kanost, M.R. (2004) Bacterial challenge stimulates innate immune responses in extra-embryonic tissues of tobacco hornworm eggs. Insect Mol. Biol. 13: 19-24. 

Dittmer, N.T., Suderman, R.J., Jiang, H., Zhu, Y.-C. Gorman, M.J., Kramer, K.J., and Kanost, M.R. (2004) Characterization of cDNAs encoding putative laccase-like multicopper oxidases and developmental expression in the tobacco hornworm, Manduca sexta, and the malaria mosquito, Anopheles gambiae. Insect Biochem. Molec. Biol. 34: 29-41.

Jiang, H., Ma, C., Lu, Z., and Kanost, M.R. (2004) β-1,3-Glucan recognition protein-2 (βGRP-2) from Manduca sexta: an acute-phase protein that binds β-1,3-glucan and lipoteichoic acid to aggregate fungi and bacteria. Insect Biochem. Molec. Biol. 34: 89-100.

Fabrick, J.A., Baker, J.E. and Kanost, M.R. (2004) Innate immunity in a pyralid moth: functional evaluation of domains from a β-1,3-glucan recognition protein. J. Biol. Chem. 279: 26605-26611.

Yu, X.-Q. And Kanost, M.R. (2004) Immulectin-2, a pattern recognition receptor that stimulates hemocyte encapsulation and melanization in the tobacco hornworm, Manduca sexta. Dev. Comp. Immunol. 9: 891-900.

Fabrick, J.A., Kanost, M.R. and Baker, J.E. (2004) RNAi-induced silencing of embryonic tryptophan oxygenase in the pyralid moth, Plodia interpunctella. J. Insect Sci. 4:15, Available online: insectscience.org/4.15.

Kanost, M.R, Jiang, H., and Yu, X.-Q. (2004) Innate immune responses of a Lepidopteran insect,Manduca sexta. Immunological Reviews. 198: 97-105.

Arakane, Y., Hogenkamp, D., Zhu, Y.C., Specht, C.A., Beeman, R.W., Kramer, K.J., Kanost, M.R., and Muthukrishnan, S. (2004) Characterization of two chitin synthase genes of the red flour beetle,Tribolium castaneum, and alternate exon usage in one of the genes during development. Insect Biochem. Mol. Biol. 34: 291-304.

Levin, D., Breuer, L.N., Zhuang, S., Anderson, S.A., Nardi, J.B. and Kanost, M.R. (2005) A hemocyte-specific integrin required for hemocytic encapsulation in the tobacco hornworm, Manduca sexta. Insect Biochem. Mol. Biol. 35: 369-380.

Tong, Y. and Kanost, M.R. (2005) Manduca sexta serpin-4 and serpin-5 inhibit the prophenoloxidase activation pathway. cDNA cloning, protein expression, and characterization. J. Biol. Chem. 280: 14923-14931

Tong, Y., Jiang, H. and Kanost, M.R. (2005) Identification of plasma proteases inhibited byManduca sexta serpin-4 and serpin-5 and their association with components of the prophenol oxidase activation pathway. J. Biol. Chem. 280: 14932-14942.

Barillas-Mury, C., Paskewitz, S., and Kanost, M.R. (2005) Immune responses of vectors. In The Biology of Disease Vectors (Marquardt, W.C., Black, W.C., Freier, J., Hagedorn, H., Hemingway, J., Higgs, S., James, A.A., Kondratieff, B., Moore, C.G. eds.) Elsevier/Academic Press. pp. 363-376.

Kanost, M.R. and Clarke, T. (2005) Proteases. In “Comprehensive Molecular Insect Science” (L.I. Gilbert, K. Iatrou, S. Gill eds) Elsevier.Vol. 4, pp. 247-266.

Arakane, Y., Muthukrishnan, S., Kramer, K.J., Specht, C.A., Tomoyasu, Y., Lorenzen, M.D., Kanost, M.R. and Beeman, R.W. (2005) The Triboliumchitin synthase Genes TcCHS1 andTcCHS2 are specialized for synthesis of epidermal cuticle and midgut peritrophic membrane, respectively. Insect Mol.Biol. 14: 453–463.

Hogenkamp, D.G., Arakane, Y., Zimoch, L., Merzendorfer, H., Kramer, K.J., Beeman, R.W., Kanost, M.R., Specht, C.A. and Muthukrishnan, S. (2005) Chitin synthase genes in Manduca sexta:Characterization of a gut-specific transcript and differential tissue expression of alternately spliced mRNAs during development. Insect Biochem. Molec. Biol. 35: 529–540.

Nardi, J. B., Zhuang, S., Pilas, B., Bee, C. M., Kanost, M. R., (2005). Clustering of adhesion receptors following exposure of insect blood cells to foreign surfaces. J. Insect Physiol. 51: 555-564.

Shen, Z., Pappan, K., Mutti, N.S., He, Q.-J., Denton, M., Zhang, Y., Kanost, M.R., Reese, J.C., and Reeck, G.R. (2005) Pectinmethylesterase from the rice weevil, Sitophilus oryzae: cDNA isolation and sequencing, genetic origin, and expression of the recombinant enzyme. J. Insect Science 5:21.

Arkane, Y., Muthukrishnan, S., Beeman, R.W., Kanost, M.R., and Kramer, K.J. (2005) Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning. Proc. Natl. Acad. Sci. USA, 102: 11337–11342.

Jiang, H., Wang, Y., Gu, Y., Guo, X., Zou, Z., Scholz, F., Trenczek, T.E. and Kanost, M.R. (2005) Molecular identification of a bevy of serine proteinases in Manduca sexta hemolymph. Insect Biochem. Molec. Biol. 35: 931-943.

Eichler, C., Lomakin, J., Arakane, Y., Kramer, K. J., Kanost, M. R., Gehrke, S. H (2005) “Insect Cuticle as a Biomimetic Material” in Advances in Bionanotechnology, N. A. Peppas and J. Z. Hilt, eds. pp. 44-48, AIChE, NY.

Nardi, J.B., Pilas, B., Bee, C.M., Garsha, K., Zhuang, S., and Kanost, M.R. (2006) Neuroglian-positive plasmatocytes and the initiation of hemocyte attachment to foreign surfaces. Dev. Comp. Immunol. 30: 447-462.

Suderman, R. J., Dittmer, N.T., Kramer, K.J. and Kanost, M.R. (2006) Model reactions for insect cuticle sclerotization: Cross-linking of recombinant cuticular proteins upon their laccase-catalyzed oxidative conjugation with catechols. Insect Biochem. Molec. Biol. 36: 353–365.

Evans, J.D., Aronstein. K., Chen, Y.P., Hetru, C., Imler, J-L., Jiang, H., Kanost, M.R., Thompson, G., Zhou, Z. Hultmark, D. (2006) Immune pathways and defense mechanisms in honey bees, Apis mellifera. Insect Molecular Biology 15: 645-656.

Zou, Z., Lopez, D. Kanost, M.R., Evans, J. and Jiang, H. (2006) Comparative analysis of serine protease-related genes in the honey bee genome: possible involvement in embryonic development and innate immunity. Insect Molecular Biology 15: 603–614.

Michel, K., Suwanchaichinda, C., Morlais, I., Lambrechts, L., Cohuet, A., Awono-Ambene, P.H., Simard, F., Fontenille, D., Kanost, M.R. and Kafatos, F.C. (2006) Increased melanizing activity in An. gambiae due to SRPN2 depletion does not affect development of Plasmodium falciparum. Proc. Natl Acad Sci. USA. 103: 16858-16863.

The Honey Bee Genome Sequencing Consortium (2006) Insights into social insects from the genome of the honey bee Apis mellifera. Nature 443: 931-949.

Zhuang, S., Kelo. L., Nardi, J.B., and Kanost, M.R. (2007) Neuroglian on hemocyte surfaces is involved in homophilic and heterophilic interactions of the innate immune system of Manduca sexta. Dev. Comp. Immunol. 31: 1159-1167.

Gorman, M.J., Wang, Y., Jiang, H., and Kanost, M.R. (2007) Manduca sexta hemolymph proteinase 21 activates prophenoloxidase activating proteinase 3 in an insect innate immune response proteinase cascade. J. Biol. Chem. 282: 11742-11749.

Waterhouse, R.M., Xi, Z.Y., Kriventseva, E., Meister, S., Alvarez, K.S., Bartholomay, L.C., Barillas-Mury, C., Bian, G., Blandin, S., Christensen, B.M., Dong, Y., Jiang, H., Kanost M.R., Koutsos, A.C., Levashina, E.A., Li, J., Ligoxygakis, P., MacCallum, R., Mayhew, G.F., Mendes, A., Michel, K., Osta, M., Paskewitz, S., Shin, S.W., Vlachou, D., Wang, L., Wei, W., Zheng, L., Zou, A., Severson, D.W., Raikhel, A.S., Kafatos, F.C., Dimopoulos, G., Zdobnov, E., and Christophides, G.K. (2007) Evolutionary dynamics of immune-related genes and pathways in disease vector mosquitoes. Science316: 1738-1743.

Kanost, M.R. (2007) Serpins in a Lepidopteran Insect, Manduca sexta. In: The Serpinopathies: Molecular and Cellular Aspects of Serpins and their Disorders, (G,A. Silverman, ed.) World Scientific Publishing Co., pp. 229-242

Zhuang, S., Kelo. L., Nardi, J.B., and Kanost, M.R. (2007) An integrin-tetraspanin interaction required for cellular innate immune responses of an insect, Manduca sexta. J. Biol. Chem. 282: 22563-22572.

Miyaji, T., Kouzuma, Y., Yaguchi, J., Matsumoto, R., Kanost, M.R., Kramer, K.J., Yonekura. M. (2007) Purification of a cysteine protease inhibitor from larval hemolymph of the tobacco hornworm (Manduca sexta) and functional expression of the recombinant protein. Insect Biochem. Molec. Biol. 37: 960-968.

Sotelo-Mundo, R.R., Lopez-Zavala, A.A., Garcia-Orozco, K.D., Arvizu-Flores, A.A., Velazquez-Contreras, E.F., Valenzuela-Soto, E.M., Rojo-Dominguez, A. And Kanost, M.R. (2007) The lysozyme from insect (Manduca sexta) is a cold-adapted enzyme. Protein and Peptide Letters 14: 774-778.

Gorman, M.J., An, C. and Kanost, M.R. (2007) Characterization of tyrosine hydroxylase from Manduca sexta. Insect Biochem. Molec. Biol. 37: 1327-1337.

Zhuang, S., Kelo. L., Nardi, J.B., and Kanost, M.R. (2008) Multiple alpha integrin subunits are involved in cell-mediated responses of the Manduca immune system. Dev. Comp. Immunol. 32: 365–379.

Ameri, M., Wang, X., Wikerson, M.J., Kanost, M.R., and Broce, A.B. (2008) An immunoglobulin binding protein (Antigen 5) of the stable fly (Diptera: Muscidae) salivary gland stimulates bovine immune responses. J. Med. Entomol. 45: 94-101.

Kanost, M.R. and Gorman, M.G. (2008) Phenoloxidases in insect immunity. In: Insect Immunology (N. Beckage, ed.) Academic Press/Elsevier, San Diego, pp. 69-96. 

Yu, X.-Q. and Kanost, M.R. (2008) Activation of Lepidopteran insect innate immune responses by C-type immulectins. In: Animal lectins: A Functional View (H.A. Ahmed and G.R. Vasta, eds.) CRC Press (Taylor and Francis), pp. 383- 395.

Gorman, M.J., Dittmer, N.T., Marshall, J.L., and Kanost, M.R. (2008) Characterization of the multicopper oxidase gene family in Anopheles gambaie. Insect Biochem. Mol. Biol. 38: 817-824.

Suwanchaichinda, C. and Kanost, M.R. (2009) The serpin gene family in Anopheles gambaie. Gene 442: 47–54.

Dittmer, N.T., Gorman, M.J. and Kanost, M.R. (2009) Comparisons between an endogenous and recombinant laccase-like multicopper oxidase from the insect Manduca sexta. Insect Biochem. Mol. Biol. 39: 596–606.

Arakane, Y., Lomakine, J., Beeman, R.W., Muthukrishnan, S., Gehrke, S.H., Kanost, M.R. and Kramer, K.J. (2009) Molecular and functional analyses of amino acid decarboxylases involved in cuticle tanning in Tribolium castaneum. J. Biol. Chem. 284: 16584-16594.

An, C., Ishibashi, J., Ragan, E.J., Jiang, H. and Kanost, M.R. (2009) Functions of Manduca sexta hemolymph proteinases HP6 and HP8 in two innate immune pathways. J. Biol. Chem. 284: 19716-19726.

Wang, X., Ribeiro. J.M.C., Broce, A.B., Wilkerson, M.J. and Kanost, M.R. (2009) An insight into the transcriptome and proteome of the salivary gland of the stable fly, Stomoxys calcitrans. Insect Biochem. Mol. Biol. 39: 607-614.

Ragan, E.J., An, C., Jiang, H. and Kanost, M.R. (2009) Roles of hemolymph proteins in antimicrobial defences of Manduca sexta. In: Insect Infection and Immunity. (S. Reynolds and J. Rolff, eds.) Oxford University Press, pp. 34-48.

Kanost, M.R. and Nardi, J.B (2009) Innate immune responses of Manduca sexta. In: Molecular Biology and Genetics of Lepidoptera. (M.R. Goldsmith and F. Marec, eds.) CRC Press, pp. 271-291.

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2010s

An, C., Jiang, H. and Kanost, M.R. (2010) Proteolytic activation and function of the cytokine Spätzle in innate immune response of a lepidopteran insect, Manduca sexta. FEBS J.277: 148–162.

Rayaprolu, S., Wang, Y., Kanost, M.R., Hartson, S. and Jiang, H. (2010) Functional analysis of four processing products from multiple precursors encoded by a lebocin-related gene from Manduca sexta. Dev. Comp. Immunol. 34:638-647.

Suderman, R.J., Dittmer, N.D., Kramer, K.J. and Kanost, M.R. (2010) Model reactions for insect cuticle sclerotization: participation of amino groups in the cross-linking of Manduca sexta cuticle protein MsCP36. Insect Biochem. Mol. Biol. 40:252-258.

Arakane, Y., Dittmer, N.T., Kramer, K.J., Muthukrishnan, S., Beeman, R.W. and Kanost, M.R. (2010) Identification, mRNA expression and partial functional analysis of the yellow family genes in Tribolium castaneum. Insect Biochem. Mol. Biol. 40:259-266.

Ragan, E.J. An, C., Yang, C. and Kanost, M.R. (2010) Analysis of mutually-exclusive alternatively spliced serpin-1 isoforms and identification of serpin-1 proteinase complexes in Manduca sexta hemolymph. J. Biol. Chem. 285: 29642-29650.

An, C. and Kanost, M.R. (2010) Manduca sexta serpin-5 regulates prophenoloxidase activation and the Toll signaling pathway by inhibiting hemolymph proteinase HP6. Insect Biochem. Mol. Biol. 40:683-689.

Dittmer, N.T. and Kanost, M.R. (2010) Insect multicopper oxidases: diversity, properties, and physiological roles. Insect Biochem. Mol. Biol. 40: 179-188.

Miyaji, T., Murayama, S., Kouzuma, Y., Kimura, N., Kanost, M.R., Kramer, K.J. and Yonekura, M. (2010) Molecular cloning of a multidomain cysteine protease and protease inhibitor precursor gene from the tobacco hornworm (Manduca sexta) and functional expression of the cathepsin-f like cysteine protease domain. Insect Biochem. Mol. Biol. 40: 835-846.

Lomakin, J., Arakane, Y. Kramer, K.J., Beeman, R.W., Kanost, M.R. and Gehrke, S.H. (2010) Mechanical properties of elytra from Tribolium castaneum wild—type and body color mutant strains. J. Insect Physiol. 56: 1901-1906.

Zhu, Y., Ragan, E.J. and Kanost, M.R. (2010) Leureptin: a soluble, extracellular leucine-rich repeat protein from Manduca sexta that binds lipopolysaccharide. Insect Biochem. Mol. Biol. 40: 713-722.

Jiang, H., Vilcinskas, A. and Kanost, M.R. (2011) Immunity in Lepidopteran Insects. Adv. Exp. Med. Biol. 708: 181-204.

An, C., Ragan, E.J. and Kanost, M.R. (2011) Serpin-1 splicing isoform J inhibits the proSpätzle-activating proteinase HP8 to regulate expression of antimicrobial hemolymph proteins in Manduca sexta. Dev. Comp. Immunol. 35: 135-141.

An, C., Budd, A., Kanost, M.R. and Michel, K. (2011) Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes. Cell. Mol. Life Sci. 68: 1929-1939.

Terenius, O., Papanicalou, A., Garbutt, J.S., Eleftherianos, I., Huvenne, H., Albrechtsen, M., An, C., Aymeric, J.-L., Barthel, A., Bebas, P., Bitra, K., Bravo, A., Chevalier, F., Collinge, D.P., Crava, C.M., de Maagda, R.A., Duvic, B., Erlandson, M., Faye, I., Felföldi, G., Fujiwara, H., Futahashi, R., Gandhe, A.S., Gatehouse, H.S., Gatehouse, L.N., Giebultowicz, J., Gómez, I., Grimmelikhuijzen, C.J., Groot, A.T., Hauser, F., Heckel, D.G., Hegedus, D.D., Hrycaj, S., Huang, L., Hull, J.J., Iatrou, K., Iga, M., Kanost, M.R., Kotwica, J., Li, C., Li, J., Lundmark, M., Matsumoto, S., Meyering-Vos, M., Millichap, P.J., Monteiro, A., Mrinal, N., Nagaraju, J., Niimi, T., Nowara, D., Ohnishi, A., Oostra, V., Ozaki, K., Papakonstandinou, M., Popadic, A., Rajam, M.V., Saenko, S., Simpson, R.M., Soberón, M., Strand, M.R., Tomita, S., Toprak, U., Wang, P., Wee, C.W., Whyard, S., Zhang, W., ffrench-Constant, R.H., Herrero, S., Gordon, K., Swevers, L., and Smagghe, G. (2011) RNA interference in Lepidoptera: an overview of successful and unsuccessful studies and implications for experimental design. J. Insect Physiology. 57: 231–245.

Lomakin, J., Huber, P.A., Eichler, C., Arakane, Y., Kramer, K.J., Beeman, R.W., Kanost, M.R. and Gehrke, S.H. (2011) Mechanical properties of the beetle elytron, a biological composite material. Biomacromolecules. 12: 321-335.

An, C., Lovell, S., Kanost, M.R., Battaile, K.P. and Michel, K. (2011) Crystal structure of native Anopheles gambiae serpin-2, a negative regulator of melanization in mosquitoes. Proteins: Structure, Function and Bioinformatics. 79: 1999-2003.

Kanost, M.R. and Clem, R.J. (2012) Insect Proteases. In “Insect Molecular Biology and Biochemistry” (L.I. Gilbert, ed) Elsevier, New York, pp. 346-364.

Dittmer, N.T., Hiromasa, Y., Tomich, J.M., Lu, N., Beeman, R.W., Kramer, K.J. and Kanost, M.R. (2012) Proteomic and transcriptomic analyses of rigid and membranous cuticles from the elytra and hindwings of the red flour beetle, Tribolium castaneum.Journal of Proteome Research. 11, 269-278.

Gorman, M.J., Sullivan, L.I., Nguyen, T.D.T., Dai, H., Dittmer, N.T., Syed, L.U., Li, J., Hua, D.H., and Kanost, M.R. (2012) Kinetic properties of alternatively spliced isoforms of laccase-2 from Tribolium castaneum and Anopheles gambiae.Insect Biochemistry and Molecular Biology 42,193-202.

Arakane, Y., Lomakin, J., Gehrke, S.H., Hiromasa, Y., Tomich, J.M., Muthukrishnan, S., Beeman, R.W., Kramer, K.J., and Kanost, M.R. (2012) Formation of rigid, non-flight forewings (elytra) of a beetle requires two major cuticular proteins.PLoS Genet 8(4): e1002682. doi:10.1371/journal.pgen.1002682

Lang, M., Kanost, M.R. and Gorman, M.J. (2012) Multicopper oxidase-3 is a laccase associated with the peritrophic matrix and malpighian tubules of Anopheles gambiae.PLoS ONE 7(3):e33985. doi:10.1371/journal.pone.0033985.

Lang, M., Braun, C.L., Kanost, M.R. and Gorman, M.J. (2012) Multicopper oxidase-1 is a ferroxidase essential for iron homeostasis in Drosophila melanogaster. Proc. Natl. Acad Sci. 109: 13,337-13,342.

Prasain, K., Nguyen, T.D.T., Gorman, M.J., Barrigan, L., Peng, Z., Kanost, M.R., Syed, L.U., Zhu, K.Y. and Hua, D.H. (2012) Redox potentials, laccase oxidation, and antilarval activities of substituted phenols.Bioorganic and Medicinal Chemistry 20: 1679–1689.

Christen, J.M., Hiromasa, Y., An, C., and Kanost, M.R. (2012) Identification of plasma proteinase complexes with serpin-3 in Manduca sexta. Insect Biochem. Mol. Biol. In press.

 

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