Michael Kanost, Ph.D., University Distinguished Professor
Areas of specialty
- Insect biochemistry and molecular biology
- Structure and function of hemolymph serine proteinases, proteinase inhibitors, and antibacterial response proteins from insects
- Biochemistry of the insect cuticle
- Function of insect multicopper oxidases
- Manduca sexta genome sequence
My laboratory is investigating proteins present in the hemolymph (blood) of insects, with special interest in the proteins' functions in the insect immune system. We are studying plasma proteins, including prophenoloxidase, serine proteases, protease inhibitors from the serpin superfamily, and proteins that bind to microbial polysaccharides. The long range goal is to understand the biochemical and cellular processes by which insect immune systems recognize and respond to pathogens and parasites. We also investigate the biochemistry of cuticle proteins and their roles in determining mechanical properties of insect exoskeletons. Our laboratory is helping to coordinate the annotation of the recently sequenced genome of the tobacco hornworm, Manduca sexta.
Takahashi, D., Dai, H., Hiromasa, Y., Krishnamoorthi, R., and Kanost, M.R. (2014) Self-association of an insect β-1,3 glucan recognition protein upon binding laminarin stimulates prophenoloxidase activation as an innate immune response.J. Biol. Chem. 289:28399-28410
Peng, Z., Green, P.G., Kanost, M.R., and Gorman, M.J. (2014) A multicopper oxidase-related protein plays a role in insect molting, life span and ovary development. PloS ONE, in press.
Noh, M.Y., Kramer, K.J., Muthukrishnan, S., Kanost, M.R., Beeman, R.W., and Arakane, Y. (2014) Two major cuticular proteins are required for assembly of horizontal laminae and vertical pore canals in rigid cuticle of Tribolium castaneum.Insect Biochem. Mol. Biol. 53, 22-29.
Dai, H., Hiromasa, Y., Takahashi, D., Vandervelde, D., Fabrick, J.A., Kanost, M.R. and Krishnamoorthi, R. (2013) An initial event in insect innate immune response: Structural and biological studies of interactions between β-1,3-glucan and the N-terminal domain of β-1,3-glucan recognition protein.Biochemistry 52:161-170.
Suwanchaichinda, C., Ochieng, R., Zhuang, S., and Kanost, M.R. (2013) Manduca sexta serpin-7, a putative regulator of hemolymph prophenoloxidase activation.Insect Biochem. Mol. Biol. 43:555-561.
Knorr, E., Bingsohn, L., Kanost, M.R., and Vilcinskas, A. (2013) Tribolium castaneum as a model for high-throughput RNAi screening.Adv. Biochem. Eng. Biotechnol., 36:163-178.
Christen, J.M., Hiromasa, Y., An, C., and Kanost, M.R. (2012) Identification of plasma proteinase complexes with serpin-3 in Manduca sexta. Insect Biochem. Mol. Biol. 42: 946-955.
Lang, M., Braun, C.L., Kanost, M.R. and Gorman, M.J. (2012) Multicopper oxidase-1 is a ferroxidase essential for iron homeostasis in Drosophila melanogaster.Proc. Natl. Acad Sci. 109: 13,337-13,342.
Dittmer, N.T., Hiromasa, Y., Tomich, J.M., Lu, N., Beeman, R.W., Kramer, K.J. and Kanost, M.R. (2012) Proteomic and transcriptomic analyses of rigid and membranous cuticles from the elytra and hindwings of the red flour beetle, Tribolium castaneum. J. Proteome Res. 11:269-278.
Arakane, Y., Lomakin, J., Gehrke, S.H., Hiromasa, Y., Tomich, J.M., Muthukrishnan, S., Beeman, R.W., Kramer, K.J., and Kanost, M.R. (2012) Formation of rigid, non-flight forewings (elytra) of a beetle requires two major cuticular proteins.PLoS Genet 8(4): e1002682. doi:10.1371/journal.pgen.1002682
Gorman, M.J., Sullivan, L.I., Nguyen, T.D.T., Dai, H., Dittmer, N.T., Syed, L.U., Li, J., Hua, D.H., and Kanost, M.R. (2012) Kinetic properties of alternatively spliced isoforms of laccase-2 from Tribolium castaneum and Anopheles gambiae. Insect Biochem. Mol. Biol. 42:193-202.
Lang, M., Kanost, M.R. and Gorman, M.J. (2012) Multicopper oxidase-3 is a laccase associated with the peritrophic matrix and malpighian tubules of Anopheles gambiae. PLoS ONE 7(3):e33985. doi:10.1371/journal.pone.0033985.
An, C., Lovell, S., Kanost, M.R., Battaile, K.P. and Michel, K. (2011) Crystal structure of native Anopheles gambiae serpin-2, a negative regulator of melanization in mosquitoes.Proteins: Structure, Function and Bioinformatics. 79: 1999-2003.
Lomakin, J., Huber, P.A., Eichler, C., Arakane, Y., Kramer, K.J., Beeman, R.W., Kanost, M.R. and Gehrke, S.H. (2011) Mechanical properties of the beetle elytron, a biological composite material.Biomacromolecules. 12: 321-335.
An, C., Budd, A., Kanost, M.R. and Michel, K. (2011) Characterization of a regulatory unit that controls melanization and affects longevity of mosquitoes.Cell. Mol. Life Sci. 68: 1929-1939.
An, C., Ragan, E.J. and Kanost, M.R. (2011) Serpin-1 splicing isoform J inhibits the proSpätzle-activating proteinase HP8 to regulate expression of antimicrobial hemolymph proteins in Manduca sexta. Dev. Comp. Immunol. 35: 135-141.