Department of Biochemistry & Molecular Biophysics
141 Chalmers Hall
Manhattan, KS 66506
785-532-6121
785-532-7278 fax
biochem@k-state.edu



Biotechnology Core Facility
206 Burt Hall
785-532-5956
785-532-6297 fax



Biomolecular NMR Facility
37 Chalmers Hall
785-532-2345

Brian Geisbrecht, Ph.D., Professor

Complete Publication List

2000s|2010s

2010s

Stapels, DA., Ramyar, KX., Bischoff, M., Von Köckritz-Blickwede, M., Milde,r FJ., Ruyken, M., Eisenbeis, J., McWhorter, WJ., Herrmann, M., Van Kessel, KP., Geisbrecht, BV., and Rooijakkers, SH. Staphylococcus aureus secretes a unique class of neutrophil serine protease inhibitors. Proc Natl Acad Sci U S A. 2014 Aug 26. pii: 201407616.

Choudhari SP, Kramer R, Barta ML, Greenwood JC 2nd, Geisbrecht BV, Joshi SB, Picking WD, Middaugh CR, Picking WL. Studies of the conformational stability of invasion plasmid antigen B from Shigella. Protein Sci. 2013 May;22(5):666-70. doi: 10.1002/pro.2249. Epub 2013 Mar 26. PubMed PMID: 23494968; PubMed Central PMCID: PMC3649268.

Garcia BL, Summers BJ, Ramyar KX, Tzekou A, Lin Z, Ricklin D, Lambris JD, Laity JH, Geisbrecht BV. A structurally dynamic N-terminal helix is a key functional determinant in staphylococcal complement inhibitor (SCIN) proteins. J Biol Chem. 2013 Jan 25;288(4):2870-81. doi: 10.1074/jbc.M112.426858. Epub 2012 Dec 11. PubMed PMID: 23233676; PubMed Central PMCID: PMC3554951.

Barta ML, McWhorter WJ, Miziorko HM, Geisbrecht BV. Structural basis for nucleotide binding and reaction catalysis in mevalonate diphosphate decarboxylase. Biochemistry. 2012 Jul 17;51(28):5611-21. Epub 2012 Jul 6. PubMed PMID:22734632.

Bannantine JP, Lingle CK, Stabel JR, Ramyar KX, Garcia BL, Raeber AJ, Schacher P, Kapur V, Geisbrecht BV. MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease. Clin Vaccine Immunol. 2012 Jul;19(7):1083-92. doi: 10.1128/CVI.00195-12. Epub 2012 May 16. PubMed PMID: 22593240; PubMed Central PMCID: PMC3393375.

Skaff DA, Ramyar KX, McWhorter WJ, Barta ML, Geisbrecht BV, Miziorko HM. Biochemical and structural basis for inhibition of Enterococcus faecalis hydroxymethylglutaryl-CoA synthase, mvaS, by hymeglusin. Biochemistry. 2012 Jun 12;51(23):4713-22. doi: 10.1021/bi300037k. Epub 2012 May 25. PubMed PMID:22510038; PubMed Central PMCID: PMC3431454.

Adam PR, Patil MK, Dickenson NE, Choudhari S, Barta M, Geisbrecht BV, Picking WL, Picking WD. Binding affects the tertiary and quaternary structures of the Shigella translocator protein IpaB and its chaperone IpgC. Biochemistry. 2012 May 15;51(19):4062-71. doi: 10.1021/bi300243z. Epub 2012 May 1. PubMed PMID:22497344.

Bouyain S, Geisbrecht BV. Host glycan recognition by a pore forming toxin. Structure. 2012 Feb 8;20(2):197-8. doi: 10.1016/j.str.2012.01.013. PubMed PMID:22325766; PubMed Central PMCID: PMC3279720.

Barta ML, Dickenson NE, Patil M, Keightley A, Wyckoff GJ, Picking WD, Picking WL, Geisbrecht BV. The structures of coiled-coil domains from type III secretion system translocators reveal homology to pore-forming toxins. J Mol Biol. 2012 Apr 13;417(5):395-405. doi: 10.1016/j.jmb.2012.01.026. Epub 2012 Feb 1. PubMed PMID:22321794; PubMed Central PMCID: PMC3304007.

Garcia BL, Summers BJ, Lin Z, Ramyar KX, Ricklin D, Kamath DV, Fu ZQ, Lambris JD, Geisbrecht BV. Diversity in the C3b [corrected] contact residues and tertiary structures of the staphylococcal complement inhibitor (SCIN) protein family. J Biol Chem. 2012 Jan 2;287(1):628-40. doi: 10.1074/jbc.M111.298984. Epub 2011 Nov 15. Erratum in: J Biol Chem. 2012 Mar 16;287(12):9329. PubMed PMID: 22086928;PubMed Central PMCID: PMC3249117.

Garcia BL, Ramyar KX, Ricklin D, Lambris JD, Geisbrecht BV. Advances in understanding the structure, function, and mechanism of the SCIN and Efb families of Staphylococcal immune evasion proteins. Adv Exp Med Biol. 2012;946:113-33. doi: 10.1007/978-1-4614-0106-3_7. Review. PubMed PMID: 21948365; PubMed Central PMCID: PMC3422867.

Barta ML, Skaff DA, McWhorter WJ, Herdendorf TJ, Miziorko HM, Geisbrecht BV. Crystal structures of Staphylococcus epidermidis mevalonate diphosphate decarboxylase bound to inhibitory analogs reveal new insight into substrate binding and catalysis. J Biol Chem. 2011 Jul 8;286(27):23900-10. doi:
10.1074/jbc.M111.242016. Epub 2011 May 11. PubMed PMID: 21561869; PubMed Central PMCID: PMC3129171.

Sever-Chroneos Z, Krupa A, Davis J, Hasan M, Yang CH, Szeliga J, Herrmann M, Hussain M, Geisbrecht BV, Kobzik L, Chroneos ZC. Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A. J Biol Chem. 2011 Feb 11;286(6):4854-70. doi:10.1074/jbc.M110.125567. Epub 2010 Dec 1. PubMed PMID: 21123169; PubMed Central PMCID: PMC3039347.

Chen H, Ricklin D, Hammel M, Garcia BL, McWhorter WJ, Sfyroera G, Wu YQ, Tzekou A, Li S, Geisbrecht BV, Woods VL Jr, Lambris JD. Allosteric inhibition of complement function by a staphylococcal immune evasion protein. Proc Natl Acad Sci U S A. 2010 Oct 12;107(41):17621-6. doi: 10.1073/pnas.1003750107. Epub 2010 Sep 27. PubMed PMID: 20876141; PubMed Central PMCID: PMC2955122.

Garcia BL, Ramyar KX, Tzekou A, Ricklin D, McWhorter WJ, Lambris JD, Geisbrecht BV. Molecular basis for complement recognition and inhibition determined by crystallographic studies of the staphylococcal complement inhibitor (SCIN) bound to C3c and C3b. J Mol Biol. 2010 Sep 10;402(1):17-29. doi:10.1016/j.jmb.2010.07.029. Epub 2010 Jul 21. PubMed PMID: 20654625; PubMed Central PMCID: PMC2930029.

Barta ML, Zhang L, Picking WL, Geisbrecht BV. Evidence for alternative quaternary structure in a bacterial Type III secretion system chaperone. BMC Struct Biol. 2010 Jul 15;10:21. doi: 10.1186/1472-6807-10-21. PubMed PMID:20633281; PubMed Central PMCID: PMC2912912.

Bannantine JP, Stabel JR, Bayles DO, Geisbrecht BV. Characteristics of an extensive Mycobacterium avium subspecies paratuberculosis recombinant protein set. Protein Expr Purif. 2010 Aug;72(2):223-33. doi: 10.1016/j.pep.2010.03.019. Epub 2010 Mar 30. PubMed PMID: 20359537.

Jongerius I, Garcia BL, Geisbrecht BV, van Strijp JA, Rooijakkers SH. Convertase inhibitory properties of Staphylococcal extracellular complement-binding protein. J Biol Chem. 2010 May 14;285(20):14973-9. doi:10.1074/jbc.M109.091975. Epub 2010 Mar 19. PubMed PMID: 20304920; PubMed Central PMCID: PMC2865291.

Haspel N, Geisbrecht BV, Lambris J, Kavraki L. Multi-scale characterization of the energy landscape of proteins with application to the C3D/Efb-C complex. Proteins. 2010 Mar;78(4):1004-14. doi: 10.1002/prot.22624. PubMed PMID: 19899169; PubMed Central PMCID: PMC2827694.

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2000s

Ricklin D, Tzekou A, Garcia BL, Hammel M, McWhorter WJ, Sfyroera G, Wu YQ, Holers VM, Herbert AP, Barlow PN, Geisbrecht BV, Lambris JD. A molecular insight into complement evasion by the staphylococcal complement inhibitor protein family. J Immunol. 2009 Aug 15;183(4):2565-74. doi: 10.4049/jimmunol.0901443. Epub 2009 Jul 22. PubMed PMID: 19625656; PubMed Central PMCID: PMC2881335.

Garcia BL, Tzekou A, Ramyar KX, McWhorter WJ, Ricklin D, Lambris JD, Geisbrecht BV. Crystallization of human complement component C3b in the presence of a staphylococcal complement-inhibitor protein (SCIN). Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt 5):482-5. doi:
10.1107/S174430910901207X. Epub 2009 Apr 24. PubMed PMID: 19407382; PubMed Central PMCID: PMC2675590.

Geisbrecht BV. Staphylococcal complement inhibitors: biological functions, recognition of complement components, and potential therapeutic implications. Adv Exp Med Biol. 2008;632:221-36. Review. PubMed PMID: 19025125.

Ricklin D, Ricklin-Lichtsteiner SK, Markiewski MM, Geisbrecht BV, Lambris JD. Cutting edge: members of the Staphylococcus aureus extracellular fibrinogen-binding protein family inhibit the interaction of C3d with complement receptor 2. J Immunol. 2008 Dec 1;181(11):7463-7. PubMed PMID: 19017934; PubMed Central PMCID: PMC2673544.

Haspel N, Ricklin D, Geisbrecht BV, Kavraki LE, Lambris JD. Electrostatic contributions drive the interaction between Staphylococcus aureus protein Efb-C and its complement target C3d. Protein Sci. 2008 Nov;17(11):1894-906. doi:10.1110/ps.036624.108. Epub 2008 Aug 7. PubMed PMID: 18687868; PubMed Central PMCID: PMC2578803.

Chen H, Schuster MC, Sfyroera G, Geisbrecht BV, Lambris JD. Solution insights into the structure of the Efb/C3 complement inhibitory complex as revealed by lysine acetylation and mass spectrometry. J Am Soc Mass Spectrom. 2008 Jan;19(1):55-65. doi: 10.1016/j.jasms.2007.10.009. PubMed PMID: 18293486; PubMed  Central PMCID: PMC2775528.

Lambris JD, Ricklin D, Geisbrecht BV. Complement evasion by human pathogens. Nat Rev Microbiol. 2008 Feb;6(2):132-42. doi: 10.1038/nrmicro1824. Review. PubMed PMID: 18197169; PubMed Central PMCID: PMC2814840.

Zemla A, Geisbrecht B, Smith J, Lam M, Kirkpatrick B, Wagner M, Slezak T, Zhou CE. STRALCP--structure alignment-based clustering of proteins. Nucleic Acids Res. 2007;35(22):e150. Epub 2007 Nov 26. PubMed PMID: 18039711; PubMed Central PMCID: PMC2190701.

Hammel M, Nemecek D, Keightley JA, Thomas GJ Jr, Geisbrecht BV. The Staphylococcus aureus extracellular adherence protein (Eap) adopts an elongated but structured conformation in solution. Protein Sci. 2007 Dec;16(12):2605-17. PubMed PMID: 18029416; PubMed Central PMCID: PMC2222813.

Hammel M, Sfyroera G, Pyrpassopoulos S, Ricklin D, Ramyar KX, Pop M, Jin Z, Lambris JD, Geisbrecht BV. Characterization of Ehp, a secreted complement inhibitory protein from Staphylococcus aureus. J Biol Chem. 2007 Oct 12;282(41):30051-61. Epub 2007 Aug 15. PubMed PMID: 17699522.

Hammel M, Sfyroera G, Ricklin D, Magotti P, Lambris JD, Geisbrecht BV. A structural basis for complement inhibition by Staphylococcus aureus. Nat Immunol. 2007 Apr;8(4):430-7. Epub 2007 Mar 11. PubMed PMID: 17351618.

Wang Z, Potter BM, Gray AM, Sacksteder KA, Geisbrecht BV, Laity JH. The solution structure of antigen MPT64 from Mycobacterium tuberculosis defines a new family of beta-grasp proteins. J Mol Biol. 2007 Feb 16;366(2):375-81. Epub 2006 Nov 14. PubMed PMID: 17174329.

Xie C, Alcaide P, Geisbrecht BV, Schneider D, Herrmann M, Preissner KT, Luscinskas FW, Chavakis T. Suppression of experimental autoimmune encephalomyelitis by extracellular adherence protein of Staphylococcus aureus. J  Exp Med. 2006 Apr 17;203(4):985-94. Epub 2006 Apr 3. PubMed PMID: 16585266; PubMed Central PMCID: PMC2118278.

Hammel M, Ramyar KX, Spencer CT, Geisbrecht BV. Crystallization and X-ray diffraction analysis of the complement component-3 (C3) inhibitory domain of Efb from Staphylococcus aureus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt 3):285-8. Epub 2006 Feb 24. PubMed PMID: 16511324; PubMed Central PMCID: PMC2197172.

Geisbrecht BV, Bouyain S, Pop M. An optimized system for expression and purification of secreted bacterial proteins. Protein Expr Purif. 2006 Mar;46(1):23-32. Epub 2005 Oct 4. PubMed PMID: 16260150.

Danahy JM, Potter BM, Geisbrecht BV, Laity JH. NMR assignment of protein Rv1980c from Mycobacterium tuberculosis. J Biomol NMR. 2005 Sep;33(1):73. PubMed PMID: 16222561.

Geisbrecht BV, Nikonenko B, Samala R, Nakamura R, Nacy CA, Sacksteder KA. Design and optimization of a recombinant system for large-scale production of the MPT64 antigen from Mycobacterium tuberculosis. Protein Expr Purif. 2006 Mar;46(1):64-72. Epub 2005 Sep 16. PubMed PMID: 16216527.

Geisbrecht BV, Hamaoka BY, Perman B, Zemla A, Leahy DJ. The crystal structures of EAP domains from Staphylococcus aureus reveal an unexpected homology to bacterial superantigens. J Biol Chem. 2005 Apr 29;280(17):17243-50. Epub 2005 Feb 3. PubMed PMID: 15691839.

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