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Integrated Genomics

Integrated Genomics Facility (IGF)

Lab # 4511, 4732, 4411, 4716 Throckmorton
Plant Sciences Center
Department of Plant Pathology
Kansas State University
Manhattan, KS 66506

phone: (785) 532-1393

Integrated Genomics Facility Equipment Access

Assistance to first-time users of Integrated Genomics Facility equipment and software is available through personal training or our workshops.

 

NanoDrop One

The NanoDrop One spectrophotometer requires only 1-2 µl of a DNA, RNA, and protein sample.

The NanoDrop website

 

 

 

Plate Reader

The Synergy H1 Multi-mode Reader supports top and bottom fluorescence intensity, UV-visible absorbance and high performance luminescence detection. It is the ideal system for all the standard microplate reader applications found in life science research laboratories.

PicoGreen_Based_Quantification_IGF_Protocol

BioTek

 

Opus

The CFX Opus 96 system can generate a thermal gradient across the 96-well reaction block to support rapid optimization of real-time PCR assays. The System can capture up to five fluorophores in a single reaction. CFX Maestro software supports data acquisition, viewing, and analysis for PCR quantification, melt curve analysis, end point analysis, allelic discrimination, and gene expression analysis.

 

 

CFX96

The CFX 96 Real-Time PCR System allows up to 5-target multiplexing. The reaction volume can be as low as 10ul. It includes thermal gradient feature. No need to normalize to a passive reference dye. CFX Manager software can be used not only to visualize all run data at once, but also to perform the complete gene expression analysis.

 

 

KASP protocol

Software:

Premier Biosoft

Primer3Plus

Equipment Producer:

BioRad Laboratories

qPCR website:

Gene - Quantification

QIAgility Liquid Handling System

QIAgility Liquid Handling System (QIAGEN) enables rapid, high precision automated transfer of liquids, for as low as 1ul, into almost any tube or plate format, including 384 well plates.

QIAgility (Qiagen)