Electronic Lab Manual

Cytogenetic techniques

• Fixation
• Pretreatment
• Staining
• In situ hybridization protocols (old)
• In situ hybridization protocols (new)
  • GISH - genomic in situ hybridication [PDF]
  • MCFISH - multi-color fluorescence in situ hybridization [PDF]

Molecular methods

• Monocot DNA isolation
• DNA digestion and Southern blotting
• Prehybridization and probe labeling
• Hybridization
• Membrane washing and striping
• Solutions
• KSUD14-STS assay for tagging Lr21
• TILLING protocol

Other protocols

• Embryo rescue medium