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Wheat Genetics Resource Center

Kansas State University
Department of Plant Pathology

4024 Throckmorton
1712 Claflin Road
Manhattan, KS 66506-5502
785-532-6176 (TEL)
785-532-5692 (FAX)
wgrc@k-state.edu

Wheat Genetics Resource Center
Kansas Wheat Innovation Center

1990 Kimball Ave
Manhattan, KS 66502
785-320-4383 (TEL)

 

FIXATIVES


In general, fixed material can be stored at 4°C for several months. The following combinations are most commonly used to study plant chromosomes:

Carnoy's solution I (Farmer's solution)

Carnoy's I is the most commonly used fixative and gives good results for a large number of different species and tissues.

  • 1 part glacial acetic acid
  • 3 parts 95% or absolute (100%) ethanol

Carnoy's solution II

Carnoy's II is recommended for oily and waxy tissues to increase the penetration ability of the fixative.

  • 1 part glacial acetic acid
  • 3 parts chloroform
  • 6 parts 95% or absolute (100%) ethanol

Propionic acid–ethanol solution

This fixative is recommended especially for species with small chromosomes.

  • 1 part propionic acid
  • 3 parts 95% or absolute (100%) ethanol