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Center on Emerging and Zoonotic Infectious Diseases

Center on Emerging and Zoonotic Infectious Diseases

Contact Information
Kansas State University
Department of Diagnostic Medicine/Pathobiology
Mosier P200
1800 Denison Avenue
Manhattan, KS 66506
Phone: (785) 532-2793
Questions/Information 
Contact: shober@vet.k-state.edu 

Molecular and Cellular Biology Core

The MCB Core will provide imaging and flow cytometry services to COBRE users. MCB Core services include DNA sequencing, CRISPR technology, and cell sorting capabilities. MCB Core support for bioinformatics and handling of large DNA sequencing datasets will be provided by the KSU Bioinformatics Center. The MCB Core offers new equipment including a NextSeq and a BD FACSMelody Cell Sorter.

Services:

ItemService Charge
Gene cloning$
Site-directed mutagenesis$
Bacterial growth rate assays$
Protein overexpression$
One-step protein purification (His, GST, FLAG, MBP)$
In vitro enzyme assay development$
Standard mammalian cell culture$
Cell culture infections (BSL2)$
Transient transfection work$
Stable-transfectant selection$
CRISPR cell library construction$
CRISPR cell library screening$                 

*Schedule of Charges coming soon

Flow Cytometry Lab:

The Flow Cytometry laboratory is housed in Mosier Hall, Room K208 of the College of Veterinary Medicine Complex. This multi-user core laboratory is equipped with state of the art flow cytometry analyzers by Becton Dickinson (LSR Fortessa X-20 and FACSCalibur), and a multiplex MagPix instrument.

Hours of Operation: M – F 8 – 5 pm, Call to schedule a time on the instruments

For schedule of charges, visit: https://www.vet.k-state.edu/academics/dmp/research/flow-cytometry/

 

hardwidge2

Dr. Phil Hardwidge, MCB Core Director

 

mwangi

Dr. Waithaka Mwangi, MCB Core Associate Director