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Wheat Genetics Resource Center

Kansas State University
Department of Plant Pathology

4024 Throckmorton
1712 Claflin Road
Manhattan, KS 66506-5502
785-532-6176 (TEL)
785-532-5692 (FAX)

Wheat Genetics Resource Center
Kansas Wheat Innovation Center

1990 Kimball Ave
Manhattan, KS 66502
785-320-4383 (TEL)



Staining should be selective, and, ideally, should only stain the chromatin and not the cytoplasm. Chromatin is basophilic, whereas the proteins in the cytoplasm are acidophilic. Acidic solutions of basic stains, such as carmine, or amphoteric stains, such as orcein, permit the differential staining of chromatin. Several staining procedures are routinely used for studying plant chromosomes. Note: this list does not include in situ (ISH) and fluorescent in situ (FISH) hybridization procedures, which are covered in a separate section.