Mouse retina: Connecting cilium
Zeiss Axioplan 2 equipped with a Zeiss Axiocam HR digital camera, a fully motorized stage with mark and find software, Plan Neofluor objectives (1.25x/0.035, 10x/0.3, 20x/0.5, 40x/0.75, 40x/1.3 oil), Plan apochromat objectives (63x/1.4 oil, 100x/1.4 oil), differential contrast interference (DIC), phase contrast (ph), HBO burner, dark field, bright field, specific filter sets for proper excitation and observation of blue, green, yellow and red fluorescence, and Axiovision software with interactive measurements and 3D deconvolution modules. This microscope is currently capable of recording multichannel- fluorescence, z-stacks over time from multiple locations on a sample. The HBO light source is capable of exciting dyes in the range from 350-700 nm.
Zeiss LSM 5 PASCAL (Laser Scanning Confocal Microscope). This system is capable of exciting fluorescent markers with laser lines of 458/488/514/543/633 nm, observation of blue, green, yellow, orange and red fluorescence, single and simultaneous double fluorescence imaging, multitracking of multifluorescence signals, acquisition of image stacks, 3D visualization, time series, multiple time series, FRET, FRAP, quantitative colocalization, orthogonal slicing of 3D data, and visualization and analysis of ion concentrations.
Light Microscopy
FEI Company Nova NanoSEM 430
Nova scanning electron microscope includes low-vacuum capability, and is ideal for the diverse imaging, analytical and sample preparation demands found in research labs, semiconductor and data storage labs and fabs, industrial applications and related industries. A Schottky emitter allows for high stability emission and high current modes while the immersion lens optimizes secondary electron collection. The new Nova NanoSEM features a helix detector that delivers high resolution images in low vacuum as well as at low kV.
This system also includes an Oxford X-Max Large Area Analytical EDS silicon drift detector (SDD) (80mm²). This detector is capable of light element detection, speed of detection, increased counts, reduced analysis time, accuracy, and liquid nitrogen free analysis.
This SEM is also equipped with a JC Nabity Lithography System (EBL).
