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Herman Lab

Restriction Enzymes

Restriction enzymes recognize specific, short sequences of double-stranded DNA where they then cleave it. For more information, see any product usage sheet that accompanies a particular enzyme.

Following is a general protocol for setting up a restriction digest:

10X buffer1.5 ul
BSA (10ug/ul)0.15ul 
DNA 3.0ul 
ddH2Oto 14.5ul

*If digesting more than one sample, 
Mix, then add:

restriction enzyme0.5ul
Final Volume 15.0ul 

Mix gently. Incubate in 37°C water bath (or appropriate temperature) for 1-4hours. 
Check on an agarose gel.