Restriction enzymes recognize specific, short sequences of double-stranded DNA where they then cleave it. For more information, see any product usage sheet that accompanies a particular enzyme.
Following is a general protocol for setting up a restriction digest:
|10X buffer||1.5 ul|
*If digesting more than one sample,
Mix, then add:
Mix gently. Incubate in 37°C water bath (or appropriate temperature) for 1-4hours.
Check on an agarose gel.