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Wheat Genetics Resource Center

Kansas State University
Department of Plant Pathology

4024 Throckmorton
1712 Claflin Road
Manhattan, KS 66506-5502
785-532-6176 (TEL)
785-532-5692 (FAX)
wgrc@k-state.edu

Wheat Genetics Resource Center
Kansas Wheat Innovation Center

1990 Kimball Ave
Manhattan, KS 66502
785-320-4383 (TEL)

 

IN SITU HYBRIDIZATION PROTOCOLS

The first three protocols describe isolation and purification of probe (plasmid) and plant genomic DNA, and the preparation of genomic blocking DNA. Other protocols describe details of chromosome preparation and in situ hybridization . The last protocol describes a modified N-banding technique that can be combined with in situ hybridization for simultaneous chromosome identification and mapping.