• Plant Pathology Department
• Wheat Page at KSU
• KSU Research & Extension
• GrainGenes
• Kansas Wheat Commission

PHYSICAL MAPPING OF THE WHEAT D GENOME

BAC Libraries

 

Library Code	Species	Variety	Chromosome	Genome fraction (%)	Number of Clones	Mean insert size	Coverage
TaaCsp146DhA	T. aestivum	Chinese Spring	1D, 4D, 6D	11.6%	87,168	85 kb	3.4 x
TaaCsp146DhB	T. aestivum	Chinese Spring	1D, 4D, 6D	11.6%	148,224	102 kb	6.9 x
TaaCsp146DhC	T. aestivum	Chinese Spring	1D, 4D, 6D	11.6%	138,240	116 kb	7.4 x
TaaCsp146DeA	T. aestivum	Chinese Spring	1D, 4D, 6D	11.6%	26,112	110 kb	1.3 x

* TaaCsp146DhA is not used for D-Genome project due to replication issues.

 

BAC LIBRARY DESCRIPTION

TaaCsp146DhA:

 

LIBRARY CODE	TaaCsp146DhA
SPECIES, CULTIVAR	Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY	1D, 4D, 6D
DATE OF CONSTRUCTION	2003
PLACE OF ORIGIN	Evry, France
CLONING SITE	HindIII
NUMBER OF CLONES	87,168
NUMBER OF PLATES	227 (No. 0001 - 0227)
NUMBER OF LIGATIONS	4
AVERAGE INSERT SIZE	85 kb
EMPTY CLONES	2%
PURITY OF THE SORTED FRANCTION	91%
GENOME/CHROMOSOME COVERAGE	3.4 x
VECTOR	pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE	Chloramphenicol (12.5 ug/ml)
HOST	Escherichia coli DH10B

Back to Top

TaaCsp146DhB:

 

LIBRARY CODE	TaaCsp146DhB
SPECIES, CULTIVAR	Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY	1D, 4D, 6D
DATE OF CONSTRUCTION	2008
PLACE OF ORIGIN	Olomouc, Czech Republic
CLONING SITE	HindIII
NUMBER OF CLONES	148,224
NUMBER OF PLATES	386 (No. 0228 - 0613)
NUMBER OF LIGATIONS	5
AVERAGE INSERT SIZE	102 kb
EMPTY CLONES	2%
PURITY OF THE SORTED FRANCTION	91%
GENOME/CHROMOSOME COVERAGE	6.9x
VECTOR	pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE	Chloramphenicol (12.5 ug/ml)
HOST	Escherichia coli DH10B

Back to Top

TaaCsp146DhC:

 

LIBRARY CODE	TaaCsp146DhC
SPECIES, CULTIVAR	Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY	1D, 4D, 6D
DATE OF CONSTRUCTION	2008
PLACE OF ORIGIN	Olomouc, Czech Republic
CLONING SITE	HindIII
NUMBER OF CLONES	138,240
NUMBER OF PLATES	360 (No. 0614 - 0973)
NUMBER OF LIGATIONS	3
AVERAGE INSERT SIZE	116 kb
EMPTY CLONES	0.5%
PURITY OF THE SORTED FRANCTION	91%
GENOME/CHROMOSOME COVERAGE	7.4x
VECTOR	pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE	Chloramphenicol (12.5 ug/ml)
HOST	Escherichia coli DH10B T1

Back to Top

TaaCsp146DeA:

 

LIBRARY CODE	TaaCsp146DeA
SPECIES, CULTIVAR	Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY	1D, 4D, 6D
DATE OF CONSTRUCTION	2008
PLACE OF ORIGIN	Olomouc, Czech Republic
CLONING SITE	EcoRI
NUMBER OF CLONES	26,112
NUMBER OF PLATES	68 (No. 0001 - 0068)
NUMBER OF LIGATIONS	3
AVERAGE INSERT SIZE	110 kb
EMPTY CLONES	2%
PURITY OF THE SORTED FRANCTION	90%
GENOME/CHROMOSOME COVERAGE	1.3x
VECTOR	pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE	Chloramphenicol (12.5 ug/ml)
HOST	Escherichia coli DH10B

Back to Top