Skip to the content

Kansas State University

PHYSICAL MAPPING OF THE WHEAT D GENOME


BAC Libraries

Library code
Species
Cultivar
Chromosome
Genome fraction (%)
Number of clones
Mean insert size
Coverage
T. aestivum
Chinese Spring
1D, 4D, 6D
11.6%
87,168
85 kb
3.4 x
T. aestivum
Chinese Spring
1D, 4D, 6D
11.6%
148,224
102 kb
6.9 x
T. aestivum
Chinese Spring
1D, 4D, 6D
11.6%
138,240
116 kb
7.4 x
T. aestivum
Chinese Spring
1D, 4D, 6D
11.6%
26,112
110 kb
1.3 x

* TaaCsp146DhA is not used for D-Genome project due to replication issues.

BAC LIBRARY DESCRIPTION

TaaCsp146DhA:

LIBRARY CODE
TaaCsp146DhA
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
1D, 4D, 6D
DATE OF CONSTRUCTION
2003
PLACE OF ORIGIN
Evry, France
CLONING SITE
HindIII
NUMBER OF CLONES
87,168
NUMBER OF PLATES
227 (No. 0001 - 0227)
NUMBER OF LIGATIONS
4
AVERAGE INSERT SIZE
85 kb
EMPTY CLONES
2%
PURITY OF THE SORTED FRANCTION
91%
GENOME/CHROMOSOME COVERAGE
3.4 x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B

Back to Top

TaaCsp146DhB:

LIBRARY CODE
TaaCsp146DhB
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
1D, 4D, 6D
DATE OF CONSTRUCTION
2008
PLACE OF ORIGIN
Olomouc, Czech Republic
CLONING SITE
HindIII
NUMBER OF CLONES
148,224
NUMBER OF PLATES
386 (No. 0228 - 0613)
NUMBER OF LIGATIONS
5
AVERAGE INSERT SIZE
102 kb
EMPTY CLONES
2%
PURITY OF THE SORTED FRANCTION
91%
GENOME/CHROMOSOME COVERAGE
6.9x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B

Back to Top

TaaCsp146DhC:

LIBRARY CODE
TaaCsp146DhC
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
1D, 4D, 6D
DATE OF CONSTRUCTION
2008
PLACE OF ORIGIN
Olomouc, Czech Republic
CLONING SITE
HindIII
NUMBER OF CLONES
138,240
NUMBER OF PLATES
360 (No. 0614 - 0973)
NUMBER OF LIGATIONS
3
AVERAGE INSERT SIZE
116 kb
EMPTY CLONES
0.5%
PURITY OF THE SORTED FRANCTION
91%
GENOME/CHROMOSOME COVERAGE
7.4x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B T1

Back to Top

TaaCsp146DeA:

LIBRARY CODE
TaaCsp146DeA
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
1D, 4D, 6D
DATE OF CONSTRUCTION
2008
PLACE OF ORIGIN
Olomouc, Czech Republic
CLONING SITE
EcoRI
NUMBER OF CLONES
26,112
NUMBER OF PLATES
68 (No. 0001 - 0068)
NUMBER OF LIGATIONS
3
AVERAGE INSERT SIZE
110 kb
EMPTY CLONES
2%
PURITY OF THE SORTED FRANCTION
90%
GENOME/CHROMOSOME COVERAGE
1.3x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B

Back to Top