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Kansas State University

IWGSC: INTEGRATED GENETIC AND PHYSICAL MAP OF CHROMOSOME 3A OF WHEAT


BAC Libraries

 

Library Code
Species
Variety
Chromosome
Genome fraction (%)
Number of Clones
Mean insert size
Coverage
T. aestivum
Chinese Spring
3AS
2.1%
55,296
80 kb
10.9 x
T. aestivum
Chinese Spring
3AS
2.1%
55,296
115 kb
15.9 x
T. aestivum
Chinese Spring
3AL
2.8%
55,296
106 kb
10.2 x
T. aestivum
Chinese Spring
3AL
2.8%
24,576
114 kb
5.2 x

 

 

BAC LIBRARY DESCRIPTION

TaaCsp3AShA:

 

LIBRARY CODE
TaaCsp3AShA
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
3AS
DATE OF CONSTRUCTION
2006
PLACE OF ORIGIN
Olomouc, Czech Republic
CLONING SITE
HindIII
NUMBER OF CLONES
55,296
NUMBER OF PLATES
144 (No. 0001 - 0144)
NUMBER OF LIGATIONS
3
AVERAGE INSERT SIZE
80 kb
EMPTY CLONES
0.5%
PURITY OF THE SORTED FRANCTION
89%
GENOME/CHROMOSOME COVERAGE
10.9 x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B

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TaaCsp3AShB:

 

LIBRARY CODE
TaaCsp3AShB
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
3AS
DATE OF CONSTRUCTION
11/2008
PLACE OF ORIGIN
Olomouc, Czech Republic
CLONING SITE
HindIII
NUMBER OF CLONES
55,296
NUMBER OF PLATES
144 (No. 0145 - 0288)
NUMBER OF LIGATIONS
2
AVERAGE INSERT SIZE
115 kb
EMPTY CLONES
1.0%
PURITY OF THE SORTED FRANCTION
91%
GENOME/CHROMOSOME COVERAGE
15.9 x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B

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TaaCsp3ALhA:

 

LIBRARY CODE
TaaCsp3ALhA
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
3AL
DATE OF CONSTRUCTION
11/2007
PLACE OF ORIGIN
Olomouc, Czech Republic
CLONING SITE
HindIII
NUMBER OF CLONES
55,296
NUMBER OF PLATES
144 (No. 0001 - 0144)
NUMBER OF LIGATIONS
4
AVERAGE INSERT SIZE
106 kb
EMPTY CLONES
5.4%
PURITY OF THE SORTED FRANCTION
87%
GENOME/CHROMOSOME COVERAGE
10.2 x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B

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TaaCsp3ALhB:

 

LIBRARY CODE
TaaCsp3ALhB
SPECIES, CULTIVAR
Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY
3AL
DATE OF CONSTRUCTION
06/2009
PLACE OF ORIGIN
Olomouc, Czech Republic
CLONING SITE
HindIII
NUMBER OF CLONES
24,576
NUMBER OF PLATES
64 (No. 0145 - 0208)
NUMBER OF LIGATIONS
2
AVERAGE INSERT SIZE
114 kb
EMPTY CLONES
1.0%
PURITY OF THE SORTED FRANCTION
88%
GENOME/CHROMOSOME COVERAGE
5.2 x
VECTOR
pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE
Chloramphenicol (12.5 ug/ml)
HOST
Escherichia coli DH10B T1

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