Broad Spectrum Resistance to Multiple Viruses in Plants 
Reference Number: 12-24
Inventors: Harold Trick, John Fellers, Jessica Rupp

Current methods to provide resistance to RNA viruses infecting wheat focus on targeting a plant gene to provide selective resistance against one specific virus. This method fails to provide the necessary long term efficacy needed to control viral diseases that result in significant yield losses, often times as high as 20-25%. Researchers at Kansas State University and USDA-ARS have developed a new method for targeting a virus gene to provide broad spectrum resistance to multiple viruses in wheat, and potentially other crops infected by RNA viruses.

This method is built on the premise that RNA viruses use host genes to complete their life cycles; therefore, when a virus enters a cell, the protein coat is removed and host proteins are then used to translate and replicate the viral RNA. With this in mind, our researchers have modified the plant so that it can limit the expression of two critical proteins used in this process. By utilizing hairpin RNA structures derived from the two critical wheat proteins, researchers have been able to induce a plant RNA protection system inside the cell that targets the RNA of these two plant genes. By reducing the expression of the wheat proteins via utilization of the siRNA, replication of the virus is prevented and resistance is created. To date, K-State and USDA researchers have produced transgenic wheat lines for each construct that are in the 5th generation, as shown below, and research shows complete resistance to both WSMV and TriMV, with potential for other viruses.

  • Targets virus gene replication, ultimately affecting the lifecycle

  • Broadens protection to multiple viruses, even those already with a host-virus system

  • Possible applicability in all cereal crops, as well as in other plants both monocot and dicots

  • Wheat production and disease resistance development

  • Private and public variety development

  • Development of virus resistant cereals and other crops

T5 eIF4E2 Lines
Event Lineage# of plantsGOI +Resistant to WSMVResistant to TriMVResistant to Co-infection
T5 eIF4G Lines
Event Lineage# of plantsGOI +Resistant to WSMVResistant to TriMVResistant to Co-infection
Patent Status:
  • US Patent Application #14/494,661 filed on September 24, 2014.

Kansas State University Research Foundation seeks to have discussions with companies that are interested in licensing and/or research collaborations.

Interested parties should contact:

Kansas State University Institute for Commercialization (KSU-IC)
2005 Research Park Circle Manhattan, KS 66502
Tel: 785-532-3900 Fax: 785-532-3909