1. K-State home
  2. »DCM
  3. »K-State News
  4. »News
  5. »2014
  6. »Researchers create better methods to detect E. coli

K-State News

K-State News
Kansas State University
128 Dole Hall
1525 Mid-Campus Dr North
Manhattan, KS 66506

785-532-2535
media@k-state.edu

Researchers create better methods to detect E. coli

Monday, June 16, 2014

       



MANHATTAN — Kansas State University diagnosticians are helping the cattle industry save millions of dollars each year by developing earlier and accurate detection of E. coli.

Lance Noll, master's student in veterinary biomedical science, Greensburg; T.G. Nagaraja, university distinguished professor of diagnostic medicine and pathobiology; and Jianfa Bai, assistant professor in the Kansas State Veterinary Diagnostic Laboratory, are leading a project to improve techniques for detecting pathogenic Shiga toxin-producing E. coli O157:H7. A U.S. Department of Agriculture Coordinated Agriculture Project grant is funding the work.

The researchers are part of a College of Veterinary Medicine team studying preharvest food safety in beef cattle. Noll has developed and validated a molecular assay that can detect and quantify major genes specific for E. coli O157.

"Developing a method to detect E. coli before it can potentially contaminate the food supply benefits the beef industry by preventing costly recalls but also benefits the consumer by ensuring the safety of the beef supply," Noll said.

The newly developed test is a molecular assay, or polymerase chain reaction, that detects bacteria based on genetic sequences, which are the bacteria's "fingerprints," Nagaraja said. The test is rapid and less labor-intensive than existing detection methods. The method can be automated and test many samples in a short period of time.

The test can be used in a diagnostic or research laboratory to accurately detect E. coli and can help with quality control in cattle facilities.

"The novelty of this test is that it targets four genes," Nagaraja said. "We are constantly working on finding better and more sensitive ways to detect these pathogens of E. coli in cattle feces."

To develop the diagnostic test, Noll and Nagaraja worked with two Kansas State University molecular biologists: Xiaorong Shi, research assistant of diagnostic medicine and pathobiology, and Bai.

"Beef cattle production is a major industry in Kansas and Kansas State University has a rich tradition in the research of beef cattle production and beef safety," Noll said. "As a graduate student in veterinary biomedical sciences, I am proud to be a member of a multidisciplinary team in the College of Veterinary Medicine that aims to make beef a safe product for the consumers."

Noll was named a winner at the 11th annual Capitol Graduate Research Summit this spring for his research project and poster, "A four-plex real-time PCR assay for the detection and quantification of Escherichia coli O157 in cattle feces."

Sources

T.G. Nagaraja
785-532-1214
tnagaraj@k-state.edu 

Lance Noll
lwnoll@k-state.edu

Pronouncer

Nagaraja is nah-gah-RAH-jah

News tip

Greensburg

Photo

Download a high-resolution photo.

Lance Noll

Lance Noll, master's student in veterinary biomedical science, presents his research poster at the Capitol Graduate Research Summit in Topeka. Noll is improving techniques for detecting pathogenic E. coli. 

Written by

Jennifer Tidball
785-532-0847
jtidball@k-state.edu

At a glance

Kansas State University diagnosticians are helping the cattle industry save millions of dollars each year by developing earlier and accurate detection of E. coli.

Notable quote

"Developing a method to detect E. coli before it can potentially contaminate the food supply benefits the beef industry by preventing costly recalls but also benefits the consumer by ensuring the safety of the beef supply."

– Lance Noll, master's student in veterinary biomedical science