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Ganging up on pathogens

Team tackles ecology of E. coli and salmonella in food animals

Understanding the ecology of two dangerous foodborne pathogens and devising ways to combat them is a big job. That’s why K-State has a team of seven researchers and six collaborators taking on E. coli 0157 and salmonella.

"It’s becoming more and more difficult to study these pathogens because you have to be a jack of all trades," said T.G. Nagaraja, professor of diagnostic medicine pathobiology at K-State’s College of Veterinary Medicine.

T.G. NagarajaNagaraja leads a research group that includes epidemiologists, molecular biologists, production animal medicine experts and feedlot nutritionists. For the past five years, Nagaraja has been leading the team on an E. coli 0157 research project that goes back more than a decade at K-State. E. coli 0157 doesn’t cause problems for livestock, but it’s zoonotic -- that is, it can be passed on to humans through the food supply.

"Our goals are fairly simple," Nagaraja said. "We want to understand the ecology of E. coli 0157 in cattle and come up with practical, on-farm intervention strategies."

The rest of the research team includes Sanjeev Narayanan, assistant professor of pathology and molecular biology; Richard Oberst, professor of microbiology; David Renter, assistant professor in epidemiology; Mike Sanderson, associate professor of epidemiology and production animal medicine; Daniel Thomson, assistant professor of feedlot production medicine; and Ludek Zurek, associate professor of entomology.

Collaborators include K-State’s Mike Apley, associate professor of production animal medicine; Jim Drouillard, professor of feedlot nutrition; Larry Hollis, professor in animal sciences and industry; Justin Kastner, assistant professor of food safety and security; and Abby Nutsch, assistant professor of food microbiology; as well as Kelly Lechtenberg, director of Midwest Veterinary Research Inc. in Oakland, Neb.

The research team is working to answer questions like why some cattle have E. coli 0157 and some don’t, and why some shed the bacteria for a longer time or at higher levels than others.

The K-State researchers also want to understand why the presence of 0157 is higher during some months, and why animals under stress shed more of the bacteria.

"If we find out answers to these questions, we can come up with intervention strategies," Nagaraja said. "The first part of the research is to look at the ecology, and the second part is to develop tests and practical intervention strategies."

For instance, Thomson is doing research with a company in Minnesota on a vaccine with antibodies that prevent the bacteria from getting iron, which they need to live. All three studies have shown a reduction in the prevalence of 0157 when the vaccine is used, Nagaraja said.

He also said that researchers are looking at what changes they could make in cattle diets that would make the animals’ digestive systems less hospitable to 0157. Because the bacteria seem to congregate in the hindgut, Nagaraja said feeding cattle a diet that will reach the hindgut and produce acid will be effective in killing 0157. He also said that probiotics -- beneficial bacteria, like those humans can get though eating yogurt -- can reduce 0157 because they out-compete the bacteria for resources.

Salmonella is one of the most common causes of gastroenteritis; it’s spread through contaminated ground beef and manure-fertilized produce. It also harms livestock. It causes bloody diarrhea in feedlot cattle and causes dairy cattle to abort. Renter’s work centers on finding out why feedlot cattle treated for other infections may show a higher rate of salmonella than do healthy cattle.

To identify the serotype of the salmonella, veterinarians and researchers currently have to send samples to a laboratory in Iowa. Narayanan is working to develop a rapid, molecular-based testing method that is more accessible.

 

Photo: T.G. Nagaraja is leading a research group studying E. coli 0157 and salmonella, two common foodborne pathogens.