Lipid Profiling Extraction Method for Yeast
Yeast can be extracted by a method similar to the one used for animal tissue (see "Extraction Method for Animal Tissue"), but usually it is necessary to apply some force to break the cells. One possibility is sonication. Another is use of a Potter-Elvehejem (Teflon-glass; driven by a drill motor or similar) homogenizer after addition of the solvent to the sample.
A lipid extraction method for yeast can also be found in the paper, Global Analysis of the Yeast Lipidome by Quantitative Shotgun Mass Spectrometry (Ejsing et al., 2009).
Wear safety glasses and protective clothing, especially when operating a Potter-Elvehejem homogenizer with the sample in solvent, because the solvent can accidentally splatter. To avoid this, start slowly.
When extraction is complete: Fill tubes with nitrogen, store in freezer; and transport to the KLRC Analytical Laboratory on dry ice. Alternatively, if you need to ship the samples, evaporate the solvent, fill the tubes with nitrogen, and ship on dry ice. You may wish to consider transferring the samples to 2 ml vials with teflon-lined caps for shipping. Please contact Mary Roth at firstname.lastname@example.org before shipping.