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Enhanced Non-endogenous siRNA Molecules and Strategy for Plant Protection

Reference Number: 09-23

Inventors: Harold Trick, Jiarui Li, Tim Todd


With annual production losses due to soybean cyst nematode alone soaring above $400 million in the U.S., new discoveries for plant protection is of most importance. Past research, as covered in the recently awarded Patent #7,803,984, demonstrates that producing small interfering RNA (siRNA) molecules with homology to specific nematode genes in plants can cause a reduction in nematodes and their progeny. Recently, further research has led to the discovery of both a method to enhance non-endogenous siRNA molecules in plants, as well as the identification of five target genes involved in the reproduction and fitness of a nematode.

Our method over-expresses either in part or full target sequences to include at least the fragment used for the RNAi hairpin together with the RNAi hairpin in the same vector. This up-regulation in both the concentration and number of individual molecule species of siRNA increases absorption of pathogenic fungus giving rise to increased plant protection. Our method also consists of enhancement through multiple targets in one construct or in a chimerical construct providing greater potential effect on the parasite or pathogen control.


The initial target is soybean cyst nematode; however, the technology could also be applicable to the following:

  • Agricultural cyst nematode species
  • Parasitic insects
  • Plant pathogenic fungi
Patent Status
  • U.S. patent #7,803,984 issued on September 28, 2010.
  • New Research - Patent protection in USA was filed in May 2012.

*Both Available for License

Kansas State University Research Foundation seeks to have discussions with companies that are interested in licensing and/or research collaborations.

Interested parties should contact:

Kansas State University Institute for Commercialization (KSU-IC)
2005 Research Park Circle Manhattan, KS 66502
Tel: 785-532-3900 Fax: 785-532-3909
E-Mail: ic@k-state.edu