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Table 1. Genetic and molecular resources that can be applied to wheat genome sequencing analysis.
TARGET
GENETIC LINKAGE MAPS
PHYSICAL GENOME RESOURCES
GENOME SEQUENCES
Hexaploid wheat, Triticum aestivum L. cv. Chinese Spring.
Genetic stocks in Chinese Spring wheat, their chromosome constitution, and number of lines available include:
Ditelosomic (20"+t"), 21;
Double-ditelosomic (20"+tS"+tL"), 20;
Ditelo–monotelosomic (20"+t"+t'), 41;
Double monosomic (19"+1'+1'), 20;
Monosomics (20"+1"), 21;
Monoisosomic (20"+i"), 7;
Trisomics (20"+1'''), 21;
Nullisomic–Tetrasomic (20"+1''''), 38;
Monosomic–Tetrasomic (20"+1'+1''''), 4;
Nullisomic (20"), 8.
Composite linkage map, based on 4,700 markers from 20 international maps, has been compiled. Markers on the map are linked electronically to the Catalog of Gene Symbols for Wheat.
Individual genetic maps are available in cMAP format through an internet interface.
  • A total of 500,000 ESTs (at least 35,000 contigs, 46,000 singletons) are recorded and approximately 6,000 are assigned to 159 bins across the 21 wheat chromosomes based on deletion and ditelsomic stocks.
  • 200,000 BAC clones arranged as contigs across the entire D-genome donor of hexaploid wheat. Approximately 1,000 ESTs assigned to BAC clones.
  • Domesticated einkorn BAC library studied in detail in rust and vernalization regions of the genome.
  • 200,000 BAC clones of the hexaploid wheat Å-genome donor T. urartu.
  • 516,000 BAC clones of durum (AABB) wheat.
  • 1,000,000 BAC clones from cvs. Chinese Spring and Norstar and 1,500,000 clones from cv. Renan plated and pooled for screening.
  • 68,000 BAC clones available from 3B; 87,168 BAC clones from 1D, 4D + 6D combined; 65,280 BAC clones from 1BS.
  • Lr10 R-gene/LMW-glutenin region on 1AS.
  • Pm3b R-gene region on 1AS.
  • Lr21 R-gene region on 1DS.
  • HMW-glutenin region on 1DL.
  • CCN R-gene region on 2DL.
  • Ph2 gene region on 3DS.
  • FHB R-gene region on 3BS.
  • Rht1 and Rht2 on 4B and 4D.
  • Q gene region on 5AL.
  • WAP2 gene region on 5AL.
  • Tsn1 gene region on 5BL.
  • Ph1 gene region on 5BL.
  • Ha locus region on 5DS.
  • Rbc S-gene region on 6 (A, B, and D).
  • Starch biosynthetic enzyme regions of 2D and 7DS.
  • Chloroplast genome.
  • Mitochondrial genome.
  • Detailed comparative genomic analyses of rice and wheat genomes to document the gaps in synteny.

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Kansas State University
December 15, 2003