|
Solution E: |
Sitosterols, N. F.
Tween 80 |
1.0 g
100 ml |
| autoclave to
sterilize and
dissolve |
|
| Solution F: |
K2HPO4 (anhydr.)
KH2PO4 (anhydr.)
NaCl
Solution E
H20
|
1.0 g
6.0 g
5.85 g
1.0 ml
to 1000 ml
|
| autoclave to
sterilize |
|
| Solution G: |
FeSO4·7 H2O
MnCl2·4 H2O
ZnSO4·7 H2O
CuSO4·5 H2O
Na2EDTA·2 H2O
H2O
| 0.69 g
0.197 g
0.287 g
0.025
g
1.86 g
to 1000
ml |
| autoclave to
sterilize |
|
| Solution H: |
Citric acid monohydrate
Tri-potassium citrate monohydrate
H2O
|
20 g
293.5 g
to 1000 ml (870 ml) |
| autoclave to
sterilize |
|
| S Medium: |
Solution F
Solution G
Solution H
Solution B (0.5M CaCl2)
Solution C (1.0M MgSO4)
|
971 ml
10.0 ml
10.0 ml
6.0 ml
3.0 ml
|