S medium (for growing worms in liquid)


Solution E: Sitosterols, N. F.
Tween 80
1.0 g
100 ml
autoclave to sterilize and dissolve



Solution F: K2HPO4 (anhydr.)
KH2PO4 (anhydr.)
NaCl
Solution E
H20
1.0 g
6.0 g
5.85 g
1.0 ml
to 1000 ml
autoclave to sterilize



Solution G: FeSO4·7 H2O
MnCl2·4 H2O
ZnSO4·7 H2O
CuSO4·5 H2O
Na2EDTA·2 H2O
H2O
0.69 g
0.197 g
0.287 g
0.025 g
1.86 g
to 1000 ml
autoclave to sterilize



Solution H: Citric acid monohydrate
Tri-potassium citrate monohydrate
H2O
20 g
293.5 g
to 1000 ml (870 ml)
autoclave to sterilize



S Medium: Solution F
Solution G
Solution H
Solution B (0.5M CaCl2)
Solution C (1.0M MgSO4)
971 ml
10.0 ml
10.0 ml
6.0 ml
3.0 ml