Restriction Enzymes


Restriction enzymes recognize specific, short sequences of double-stranded DNA where they then cleave it. For more information, see any product usage sheet that accompanies a particular enzyme.

Following is a general protocol for setting up a restriction digest:

 

10X buffer 1.5 ul
BSA (10ug/ul) 0.15ul
DNA 3.0ul
ddH2O to 14.5ul

*If digesting more than one sample,
Mix, then add:

restriction enzyme 0.5ul
Final Volume 15.0ul

Mix gently. Incubate in 37°C water bath (or appropriate temperature) for 1-4hours.
Check on an agarose gel.