Restriction enzymes recognize
specific, short sequences of double-stranded DNA where they then cleave
it. For more information, see any product usage sheet that accompanies
a particular enzyme.
Following is a general protocol
for setting up a restriction digest:
*If digesting more than one
Mix, then add:
Mix gently. Incubate in 37°C
water bath (or appropriate temperature) for 1-4hours.
Check on an agarose gel.