HERMAN LAB PROTOCOLS
WORM PROTOCOLS

a. Chemotaxis Assay
b. EMS Mutagenesis
c. Generating Males by Heat Shock
d. Genomic Prep
e. Microarray Procedure
f. Microinjections
g. Nematode Dye Filling
h. Nematode Double Dye Filling
i. PCR from Worms
j. SNIP SNP Mapping
k. Soaking RNAi
l. UV array integration
l. Worm Breeding for Super Geniuses
m. Worm Decontamination
n. Worm Freezing
o. Worm Lysis
p. Larval Antibody Staining- POP-1

MOLECULAR BIOLOGY PROTOCOLS

a. 5-minute transformation
b. Agarose Gel Electrophoresis
c. Agencourt PCR Cleanup Procedure
d. Antibiotic Usage
e. cDNA Phage Stock
f. Competent Cells
g. Cosmid Prep
h. Ethanol Precipitation
i. Freezing bacterial stocks
j. Herman Lab Media Additive List
k. Ligations
l. PCR Using Dynazyme
m. PCR Using Phusion
n. Phenol-Chloroform Extraction
o. Promega Miniprep Protocol – Old Style
p. Qiagen Cosmid Prep
q. Restriction Enzymes
r. RFLP on PCR Products
s. Sequencing Requirements
t. Standard PCR Conditions