1. K-State home
  2. »Division of Biology
  3. »Herman Lab
  4. »Protocols

Herman Lab

C. elegans Lab Protocols

WormMethods

Microscope Cleaning and Maintenance (from Zeiss)

Herman Lab Protocols:

WORM PROTOCOLS 

EMS Mutagenesis
Generating Males by Heat Shock
Genomic Prep
Microinjections
PCR from Worms
UV array integration
Worm Decontamination
Worm Freezing
Worm Lysis

MOLECULAR BIOLOGY PROTOCOLS

5-minute transformation
Agarose Gel Electrophoresis
Antibiotic Usage
Competent Cells
Ethanol Precipitation
Freezing bacterial stocks
Herman Lab Media Additive List
Ligations
PCR Using Phusion
Phenol-Chloroform Extraction
Restriction Enzymes
Standard PCR Conditions