Generating males by heat shock

Koelle Lab

  1. Set up ~6 plates with 5 L4 hermaphrodites each.
  2. Heat shock 4-6 hours at 30C.  8- 9 hours works but gives few progeny.
  3. Return to 20C.  Should get a few males per plate in the F1.
  4. When you only have a few males, it is best to set them up with an excess of L4 hermaphrodites to ensure recovering more males in the next generation.  Subsequently,  male stocks should be maintained by selecting an excess of males, along with a few L4 hermaphrodites, each generation.