Generating males by heat shock
- Set up
~6 plates with 5 L4 hermaphrodites each.
- Heat shock
4-6 hours at 30¡C. 8- 9 hours works but gives few progeny.
to 20¡C. Should get a few males per plate in the
- When you
only have a few males, it is best to set them up with an excess of L4 hermaphrodites
to ensure recovering more males in the next generation. Subsequently,
male stocks should be maintained by selecting an excess of males, along
with a few L4 hermaphrodites, each generation.