Worm Decontamination with Clorox
a plate with plenty opf eggs, wash off with M9 into a conical tube.
Eggs may stick in bacterial lawn, so gently wipe plate with spatula to loosen.
down (I use speed Ô4Õ on ancient centrifuge, for about one minute).
Resuspend in 2mls decontamination solution (see below).
sit 6-10 minutes*, flicking the tube occasionally to break up worms.
4 minutes in centrifuge.
in M9, pellet 1 minute.
supernatant, pipet eggs onto a plate with a Pasteur pipet.
- Use 10 mintes for grey slime – it is extra
M9 1.4ml 35ml