Requirements for K-State Plant Path Sequencing Facility
See specific instructions on the “On Campus Sequencing Form”
found at the website, which includes which stock primers they provide.
Template type and amount needed for reaction:
ds plasmid 0.5ug (concentration = 0.25ug/ul)
PCR product 5ng/100 bases (concentration = 2.5ng/100 bases/ul)
Samples should be dissolved
in ddH2O. Send enough samples so that they can be rerun if they don’t
Primer concentration = 5pmol/ul. Our stock solutions are 200pmol/ul,
so to make 20ul at 5pmol/ul, add 0.5ul of stock solution to 19.5ul of
Label all tubes with 6 characters max.
Print out filled in sequencing form, get a requisition from the accounting
office, and take those and your samples to the facility.