Confocal: Zeiss 700

Confocal Zeiss 700 Instrument

The confocal microscope, Carl Zeiss 700, consists of an inverted microscope outfitted with five objectives, 2.5x, 5x, 20x, 40x (1.4 NA Oil) and 40x (1.3 NA Water), four lasers, 405 (blue), 488 (cyan), 555 (green) and 635 nm (red), two fluorescence emission detectors and one transmission detector. 

The instrument is suitable for three-color co-localization with minimized spectral overlap, overlay of fluorescence onto bright-field images, fluorescence recovery after photo-bleaching (FRAP) and fluorescence Resonance Energy Transfer (FRET). 

The Carl Zeiss 700 is set up for traditional work with tissue samples mounted on slides or held in glass-bottom culture dishes.  

Access:

Projects are initiated by a meeting of the user, principal investigator, Core Director and Core Manager.  Contact Mr Joel Sanneman.  
The Core provides individual training.  
Trained users coordinate instrument use via the Scheduler.