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Dr. Stefan Bossmann

 

Professor

M.S., University of Saarland, Germany (1988)
Ph.D., University of Saarland, Germany (1991)
Postdoctoral Research Fellow, Columbia University (1991-3)
Professor, University of Karlsruhe (1993-2004)

Email: sbossman@ksu.edu
Office Phone: 785-532-6817
Lab Phone: 785-532-3090 (3rd Floor)
Lab Phone: 785-532-6672 (4th Floor)
Fax: 785-532-6666

Bossmann Group

 

 

Research Overview

The porin MspA from M. smegmatis as Building Element in Nanotechnology

The octameric porin MspA from Mycobacterium smegmatis is sufficiently stable to form a nonmembrane-supported stand-alone porin on mica surfaces. About 98% of all MspA octamers were found to stand upright on mica, with their periplasmic loop regions bound to the hydrophilic mica surface. Both, small (d=3.7 nm) and large (d=17 nm) gold nanoparticles bind to MspA, however, in different positions: small gold nanoparticles bind within the MspA pore, whereas the large gold nanoparticles bind to the upper region of MspA. These experiments demonstrate that gold nanoparticles can be positioned at different, well-defined distances from the underlying surface using the MspA pore as a template. These findings represent a significant step toward the use of electrically insulating stable proteins in combination with metal nanoparticles in supramolecular nanodevices.

Calculated heights of the complexes: small Au@MspAQ126C (left, height: 10 nm), small Au@MspAWT (middle, height: 12.5 nm), and large Au@MspAWT complexes (right, height: 26.5 nm) (with thanks to Dr. Paul Smith, KSU Chem)

 

Photochromic Systems for Optical Information Storage

We are synthesizing new photochromic styryl-quinolyl dihydroindolizine/betaine systems for optical information storage, in which both constitutional isomers show remarkably strong fluorescence. The DHI (1) undergoes a 1,5-electrocyclic reaction to betaine (2) upon irradiation with UVA light λ < 400 nm). When stabilized in a PMMA matrix, the blue betaine (2) is stable for hours at room temperature and indefinitely stable at 77K against the thermal back reaction to the DHI (1).

 

 

Irradiation of betaine (2) with λ  = 532 nm permits the photo-induced back switching process to DHI (1). The betaine (2) permits its destruction-free read-out at λ  = 645 nm when excited with λ  = 580 nm. This system has enabled the realization of a prototype for an information recording and storage system.

 

Left: VIS-absorption spectrum of the “runner” (DHI (1) in PMMA after writing with a 355nm laser beam. Right: Fluorescence spectrum from the “runner”, excitation at 532nm.

 

Selected Publications

Wang, H. W.; Shrestha, T. B.; Basel, M. T.; Dani, R. K.; Seo, G. M.; Balivada, S.; Pyle, M. M.; Prock, H.; Koper, O. B.; Thapa, P. S.; Moore, D.; Li, P.; Chikan, V.; Troyer, D. L.; Bossmann, S. H. Magnetic-Fe/Fe3O4-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages Beilstein J Nanotech 2012, 3, 444-55.

Shrestha, T. B.; Seo, G. M.; Basel, M. T.; Kalita, M.; Wang, H.; Villanueva, D.; Pyle, M.; Balivada, S.; Rachakatla, R. S.; Shinogle, H.; Thapa, P. S.; Moore, D.; Troyer, D. L.; Bossmann, S. H. Stem cell-based photodynamic therapy Photochem Photobiol Sci 2012, 11, 1251-8.

Basel, M. T.; Shrestha, T. B.; Troyer, D. L.; Bossmann, S. H. Protease-Sensitive, Polymer-Caged Liposomes: A Method for Making Highly Targeted Liposomes Using Triggered Release ACS Nano 2011, 5, 2162-75.

Rachakatla, R. S.; Balivada, S.; Seo, G.-M.; Myers, C. B.; Wang, H.; Samarakoon, T. N.; Dani, R.; Pyle, M.; Kroh, F. O.; Walker, B.; Leaym, X.; Koper, O. B.; Chikan, V.; Bossmann, S. H.; Tamura, M.; Troyer, D. L. Attenuation of Mouse Melanoma by A/C Magnetic Field after Delivery of Bi-Magnetic Nanoparticles by Neural Progenitor Cells., ACS Nano 2010, 4, 7093-7104.

Basel, M. T.; Dani, R. K.; Kang, M.; Pavlenok, M.; Chikan, V.; Smith, P. E.; Niederweis, M.; Bossmann, S. H. Direct Observation of Gold Nanoparticle Assemblies with the Porin MspA on Mica., ACS Nano 2009, 3, 462-466.

Shrestha, T. B.; Melin, J.; Liu, Y.; Dolgounitcheva, O.; Zakrzewski, V. G.; Pokhrel, M. R.; Gogritchiani, E.; Ortiz, J. V.; Turro, C.; Bossmann, S. H. New insights in the photochromic spiro-dihydroindolizine/betaine-system., Photochem. Photobiol. Sci. 2008, 7, 1449-1456.

 

Complete Publication List

Hong Kong Symposium in Honour of Prof. Po Lock Yue

My complete response to the TB discussion started on 9.11.2007 in the Kansas State Collegian - What's the Matter with Tuberculosis.pdf (click to read)