K-State Division of Biology

Chingakham Ranjit Singh

Research Assistant Professor

Ph.D., 2002, North Eastern Hill University, Shillong, India

Translational regulation of protein synthesis. Structure and function of ribosome-inactivating proteins and their possible applications in biomedicine as cytotoxic drugs. Molecular mechanisms of proteasome assembly and regulation


345 Ackert Hall

Molecular Cellular Developmental Biology Web

Selected Research Publications

Jean-pierre H. Perchellet, Elisabeth M. Perchellet, Chingakham Ranjit Singh, Meghan T. Monnett, Elizabeth Studer and Keith R. Buszek (2014) Mechanisms by Which Synthetic 6,7-Annulated-4-substituted Indole Compounds with Antiproliferative Activity Disrupt Mitosis and Block Cytokinesis in Human HL-60 Tumor Cells. Anticancer Research, International Journal of Cancer Research and Treatment (Special Issue on "New Anticancer Agents"). 2014, 34:4.

Chingakham Ranjit Singh, Scott Lovell, Nurjahan Mehzabeen, Wasimul Q. Chowdhury, Erid S. Geanes, Kevin P. Battaile and Jeroen Roelofs (2014) 1.15 Å resolution crystal structure of the Proteasome Assembly Chaperone Nas2 PDZ Domain.  Acta Crystallographica Section F. F70, 418-423

Naoki Nemoto, Tsuyoshi Udagawa, Wasimul Chowdhury, Makoto Kitabatake,  Byung-Sik Shin, Hiroyuki Hiraishi, Suzhi Wang, Chingakham Ranjit Singh, Susan J. Brown, Mutsuhito Ohno, and Katsura Asano (2013) Random mutagenesis of yeast 25S rRNA identify bases critical for 60S subunit structural integrity and function. Translation. Vol-1, Issue 2

Alina De La Mota-Peynado, Stella Y. Lee, Brianne M. Pierce, Prashant Wani, Chingakham Ranjit Singh and Jeroen Roelofs (2013) The proteasome-associated protein Ecm29 inhibits proteasomal ATPase activity and in vivo protein degradation by the proteasome. Journal of Biological Chemistry. First Published on August 30, 2013, doi:10.1074/jbc.M113.491662.

Soyeon Park, Xueming Li, Ho Min Kim, Chingakham Ranjit Singh, Geng Tian, Martin A. Hoyt, Scott Lovell, Kevin P. Battaile, Michal Zolkiewski, Philip Coffino, Jeroen Roelofs, Yifan Cheng, and Daniel Finley. 2013. Reconfiguration of the proteasome during chaperone-mediated assembly. Nature, 497, 512-516.

Chingakham Ranjit Singh, Ryosuke Watanabe, Wasimul Chowdhury, Hiroyuki Hiraishi, Marcelo J. Murai, Yasufumi Yamamoto, David Miles, Yuka Ikeda, Masayo Asano, and Katsura Asano. 2012. Sequential Eukaryotic Translation Initiation Factor 5 (eIF5) Binding to the Charged Disordered Segments of eIF4G and eIF2β Stabilizes the 48S Preinitiation Complex and Promotes Its Shift to the Initiation ModeMolecular and Cellular Biology. 2012, 32(19): 3978-3989.

Chingakham Ranjit Singh, Ryosuke Watanabe, Susan Campbell, Ronald C. Wek, Graham D. Pavitt, Mark P. Ashe, John A. Chiorini and Katsura Asano. 2011. Mechanisms of translational regulation by a human eIF5-mimic protein. Nucleic Acids Research. 2011: 1–15.

Naoki Nemoto, Chingakham Ranjit Singh, Tsuyoshi Udagawa, Suzhi Wang, Elizabeth Thorson, Zachery Winter, Takahiro Ohira, Miki Ii, Leos Valasek, Susan J. Brown, and Katsura Asano. 2010. Yeast 18S rRNA is directly involved in the ribosomal response to stringent  AUG selection during translation initiation. Journal of Biological Chemistry. 285: 32200-32212.

Ryosuke Watanabe, Marcelo Jun Murai, Chingakham Ranjit Singh, Stephanie Fox, Miki Ii and Katsura Asano. 2010. The Eukaryotic Initiation Factor (eIF) 4G HEAT Domain Promotes Translation Re-initiation in Yeast Both Dependenton and Independent of eIF4A mRNA Helicase. Journal of Biological Chemistry. 285: 21922-21933.

Mikhail Reibarkh, Yasufumi Yamamoto, Chingakham Ranjit Singh, Federico del Rio, Amr Fahrmy, Bumjun Lee, Gerhard Wagner, and Katsura Asano. 2008. Eukaryotic Initiation Factor (eIF) 1 carries two distinct eIF5-binding faces important for multifactor assembly and AUG selection. Journal of Biological Chemistry. 283: 1094-1103.

Chingakham Ranjit Singh and Katsura Asano. 2007. Localization and characterization of protein-protein interaction sites using yeast S. cerevisiae as a model organism. In “Methods in Enzymology” (J. Lorsch, ed.) 429: 140-160.

Bumjun Lee, Tsuyoshi Udagawa, Chingakham Ranjit Singh and Katsura Asano. 2007. Yeast phenotypic assays on translational control. In “Methods in Enzymology” (J. Lorsch, ed.) 429: 106-128.

Chingakham Ranjit Singh, Tsuyoshi Udagawa, Bumjun Lee, Sarah Wassink, Hui He, Yasufumi Yamamoto, James T. Anderson, Graham D. Pavitt, and Katsura Asano. 2007. Change in nutritional status modulates the abundance of critical pre-initiation intermediate complexes during translation initiation in vivo. Journal of Molecular Biology. 370: 315-330.

Chingakham Ranjit Singh, Bumjun Lee, Tsuyoshi Udagawa, Sarah S. Mohammad-Qureshi, Yasufumi Yamamoto, Graham D. Pavitt, and Katsura Asano. 2006. An eIF5/eIF2 complex antagonizes guanine nucleotide exchange by eIF2B during translation initiation. The EMBO Journal. 25: 4537–4546.

Yasufumi Yamamoto, Chingakham Ranjit Singh, Assen Marintchev, Nathan S. Hall, Ernest M. Hannig, Gerhard Wagner, and Katsura Asano. 2005. The eukaryotic initiation factor (eIF) 5 HEAT domain mediates multifactor assembly and scanning with distinct interfaces to eIF1, eIF2, eIF3, and eIF4G. Proct. Natl. Acad. Sci. USA. 102: 16164-16169.

Chingakham Ranjit Singh, Cynthia Curtis, Yasufumi Yamamoto, Nathan S. Hall, Dustin S. Kruse, Hui He, Ernst M. Hannig and Katsura Asano. 2005. Eukaryotic Translation Initiation Factor 5 is critical for integrity of the scanning preinitiation complex and accurate control of GCN4 translation. Molecular & Cellular Biology. 25: 5480 – 5491.

Chingakham Ranjit Singh, Yasufumi Yamamoto and Katsura Asano. 2004. Physical association of eukaryotic initiation factor 5 (eIF5) carboxyl terminal domain with the lysine-rich eIF2 segment strongly enhances its binding to eIF3. Journal of Biological Chemistry. 279: 49644 - 49655.

Chingakham Ranjit Singh, Hui He, Miki Ii, Yasufumi Yamamoto, and Katsura Asano. 2004. Efficient incorporation of eIF1 into the multifactor complex is critical for formation of functional ribosomal preinitiation complexes in vivo. Journal of Biological Chemistry. 279: 31910 – 31920.

Hui He, Tobias Von Der Haar, Chingakham Ranjit Singh, Miki Ii, Bin Li, Alan G. Hinnebusch, John E. G. McCarthy, and Katsura Asano. 2003. The yeast eIF4G HEAT domain interacts with eIF1 and eIF5 and is involved in stringent AUG selection. Molecular & Cellular Biology. 23: 5431 - 5445.

Ranjit Chingakham Singh, Anis Alam and Vinod Singh. 2003. Purification, characterization and chemical modification studies on a translation inhibitor protein from Luffa cylindrica. Indian J. Biochem. & Biophysics. 40: 40-50.

Anis Alam, Ranjit Chingakham Singh and Vinod Singh. 2002. Design and synthesis of hormonotoxin for selective targeting of gonadal cells. Indian. J. Exp. Biol. 40: 477-485.

Ranjit Chingakham Singh, Vinod Singh and Anis Alam. 2002. Studies on chemical modification of ovine luteinizing hormone and its subunits with different heterobifunctional cross-linking agents. Indian. J. Exp. Biol. 40: 456-466.

Ranjit Chingakham Singh, Anis Alam and Vinod Singh. 2001. Role of positive charge of lysine residue on ribosome-inactivating property of gelonin. Indian J. Biochem. & Biophysics. 38: 309-312.

Vinod Singh, Ranjit Chingakham Singh, Rajesh Kumar  Dubey and Anis Alam. 2000. Ribosome-inactivating property of gelonin is more affected to N-succinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate modification than N-succinimidyl-3-(2-pyridylthio) propionate. Indian J. Biochem. & Biophysics, 37: 155-165.

Ranjit Chingakham Singh and Vinod Singh. 2000. Current status of ribosome inactivating proteins. Indian J. Biochem. & Biophysics. 37(1): 1-5.

Vinod Singh, Ranjit Chingakham Singh and R.K. Dubey. 1999. Significance of charge on lysine residue of ovine luteinizing hormone on immunological and biological properties of the hormone. Indian J. Biochem. & Biophysics. 36(6): 398-404.

Vinod Singh and Ranjit Chingakham Singh. 1999. Modification of ovine luteinizing hormone subunits with SMPT and its effect on subunit recombination, immunological activity, receptor binding and steroidogenic activity. Indian J. Exp. Biol. 37: 849-858.

Vinod Singh, Ranjit Chingakham Singh, Rajesh K. Dubey and Anis Alam. 1999. Purification and Characterisation of  Gelonin from seeds of Gelonium multiflorum. Indian J. Biochem. & Biophysics. 36: 258-265.

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