Projects in my laboratory focus on macrophages, inflammatory cells and other white blood cells. I am interested in the function of these cells, the role these cells have in infectious diseases and the impact of space flight on these cells. Our current focus areas include:
1) Experiments are currently in progress to determine how macrophage differentiation is altered by space flight or simulated microgravity. My laboratory is using ground based models and space shuttle flights to make these determinations.
2) My group has created mice lacking various combinations of macrophage response genes (e.g. MHCII, Tlr4, CD81 and Slc11a1). We are using these mice to look at the impact of multiple gene defects to understand the host response to organisms like Pasteurella pneumotropica and Ehrlichia chaffeensis. In particular, we are attempting to understand how the host immune response controls opportunistic microorganisms.
3) We are currently attempting to understand how macrophage trafficking and function is influenced by adipose tissue using immunological, molecular and biochemical techniques.
4) Our group is working to understand which host genes are necessary for intracellular growth of E. chaffeensis using the Drosophila melanogaster as a model system.
View an article about Keith Chapes' research in Space Research, Vol. 2 No. 3, June 2003, P. 14-15, 25.
Luce-Fedrow, A., T.Von Ohlen, D. Boyle, R.R. Ganta, S.K. Chapes. 2008. Use of Drosophila S2 cells as a model for studying Ehrlichia chaffeensis infections. Appl. Environ. Microbiol. 74:1886-1891.
Potts, B.E. and S.K. Chapes. 2008. Functions of C2D macrophage cells after adoptive transfer. J. Leukocyte Biol. 83:602-609.
Potts, B.E., M. L. Hart, L.L. Snyder, D. Boyle, D. A. Mosier and S.K. Chapes. 2008. Differentiation of C2D macrophage cells after adoptive transfer. Clin. Vaccine Immunol. 15:243-252.
Xie, L., C. P. O'Reilly, S. K. Chapes and S. Mora. 2008. Adiponectin and leptin are secreted through distinct trafficking pathways in adipocytes. Biochemica, Biophysica, Acta. 1782:99-108.
R.R. Ganta, C. Cheng, E.C. Miller, B.L. McGuire, and S.K. Chapes. 2007. Differential clearance and immune responses to tick cell vs. macrophage culture-derived Ehrlichia chaffeensis in mice. Infect. Immun. 75:135-145.
Chapes, S.K. and R.R. Ganta. 2005. Mouse Infection Models for Space Flight Immunology. Adv. in Space Biol.and Medicine; Issue: Experimentation with Animal Models in Space, Ed. by G. Sonnenfeld, Elsevier, New York. pp. 81-104.
Armbrust, L., D.A. Mosier, E. Nelson, M.L. Hart, and S.K. Chapes. 2005. Correlation of results of pulmonary computed tomography and pathologic findings in mice with Pasteurella-induced pneumonia. Am. J. Vet. Res. 66:835-838.
Chapes, S.K. 2004. Lessons from Immune 1-3: What did we learn and what do we need to do in the future? J. Gravitational. Physiol. 11(2):45‑48.
Ganta, R.R., C. Cheng, M.J. Wilkerson, and S.K. Chapes. 2004. Delayed clearance of Ehrlichia chaffeensis infection in CD4+ T-cell knock out mice. Infect. Immun. 72:159-167.
Hart, M.L., D.A. Mosier, and S.K. Chapes. 2003. Toll-like receptor 4 (TLR4)-positive macrophages protect mice from Pasteurella-induced pneumonia. Infect. Immun. 71(2):663-670.
Stewart, P.W. and S.K. Chapes. 2003. Role of major histocompatibility complex class II in resistance of mice to naturally acquired infection with Syphacia obvelata. Comparative Med. 53: 70-74.
Ganta, R.R., M.J. Wilkerson, C. Cheng, A.M. Rokey, and S.K. Chapes. 2002. Persistent Ehrlichia chaffeensis infection occurs in the absence of functional MHCII genes. Infect. Immun. 70: 380-388.
Simske, S.J., T.A. Bateman, E.E. Smith, V.L. Ferguson and S.K. Chapes. 2002. Effects of major histocompatibility complex class II knockout on mouse bone mechanical properties during development. Proceed. Biomed. Sci. Instrum. 38:47-52.
Chapes, S.K., D.A. Mosier, A.D. Wright, and M.L. Hart. 2001. MHCII, Tlr4 and Nramp1 genes control host pulmonary resistance against the opportunistic bacterium Pasteurella pneumotropica. J. Leukoc. Biol. 69:381-386.
Chapes, S.K. A.D. Wright, and A.A. Beharka. 2001. Superantigen activation of macrophages. In: Staphylococcal Disease and Infection (Chapter 5), Ed by A.L. Honeyman, M. Bendinelli, H. Friedman, Kluwer Academic/Plenum Press. New York. pp. 67-91.
Wright, A.D. and S.K. Chapes. 1999. Staphylococcal enterotoxin A bound to MHC class I requires cross linking for the induction of TNF-α. Cellular Immunol. 197:129-135.
Wright, A.D. and S.K. Chapes. 1999. LPS Sensitivity in recombinant mice lacking functional alleles at MHCII, Lps and Nramp1 genes. J. Endotoxin Res. 5:297-308.