Takeo Iwamoto

Research Assistant Professor, Associate Director, Biotechnology Core Facility Sr. Scientist - Nacelle Therapeutics

The Tomich laboratory designs and characterizes synthetic peptides for potential uses as drugs or renewable biomaterials. The lab employs a number of biologic, synthetic, analytic and physical methods to make these characterizations.

B.S. 1982, Tokyo University of Pharmacy, Japan
Ph.D. 1988, Tokyo Institute of Technology

Phone: 785-532-5956
Fax: 785-532-7278
Email: iwamoto@k-state.edu
Office: 214 Burt Hall

1982-1990 Research Associate, The Jikei University School of Medicine, Tokyo, Japan.  Keiji Iriyam Supervisor.
1990-1992 Ph.D. Research Fellow, Department of Biochemistry, University of Southern California School of Medicine, Los Angeles, California.  John M. Tomich, Supervisor.
1992-1998 Assistant Director Biotechnology Core Facility, Kansas State University, Manhattan, Kansas
1999-present Research Assistant Professor of Biochemistry, Kansas State University, Manhattan, Kansas
1999-present Associate Director, Biotechnology Core Facility, Kansas State University, Manhattan, Kansas
2002-present Senior Scientist, Nacelle Therapeutics, Inc. Manhattan, Kansas

Selected Publications
 
Smith, E., Tomich, J. M., Iwamoto, T., Richards, J. H. and Feinberg, B.  (1991)  A totally synthetic histidine-2 ferredoxin:  Thermal stability and redox properties.  Biochemistry 30, 11669-11676. 

Nozawa, M., Iwamoto, T., Tokoro, T., and Eto, Y.  (1992) Novel procedure for measuring psychosine derivatives by an HPLC method.  J. Neurochem. 59, 607-609.

Montal, M. S., Iwamoto, T., Tomich, J. M. and Montal, M.  (1993)  Design, synthesis and functional characterization of a pentameric channel protein that mimics the presumed pore structure of the nicotinic cholinergic receptor.  FEBS Lett. 320, 261-266.

Montal, M. O., Bühler, L., Iwamoto, T., Tomich, J. M. and Montal, M.  (1993)  Synthetic peptides and four-helix bundle proteins as model systems for the pore-forming structure of channel proteins. Transmembrane segment M2 of the nicotinic cholinergic receptor channel is a key pore lining structure.   J. Biol. Chem., 268, 14601-07.

Reddy, L. G., Iwamoto, T., Tomich, J. M. and Montal, M.  (1993)  Synthetic peptides and four-helix bundle proteins as model systems for the pore-forming structure of channel proteins. Transmembrane segment M2 of the brain glycine receptor channel is a plausible candidate for the pore-lining structure.  J. Biol. Chem., 268, 14608-15.

Grove, A., Tomich, J. M., Iwamoto, T. and Montal, M.  (1993)  Design of a functional calcium channel protein:  Inferences about an ion channel forming motif derived from the primary structure of voltage-gated calcium channels.  Protein Sci. 2, 1918-1930. 

Montal, M. O., Reddy, L. G., Iwamoto, T., Tomich, J. M. and Montal, M.  (1994)  Identification of an ion channel-forming motif in the primary structure of CFTR, the cystic fibrosis chloride channel.  Proc. Natl. Acad. Sci. USA. 91, 1495-1499.

Iwamoto, T., Grove, A., Oblatt-Montal, M., Montal, M. and Tomich, J. M.  (1994)  Chemical synthesis and characterization of peptides and oligomeric proteins designed to form transmembrane ion channels.  Int. J. Pept. Prot. Res. 43, 597-607. 

Shi MM, Kugelman A, Iwamoto T, Tian L, Forman HJ.  Quinone-induced oxidative stress elevates glutathione and induces gamma-glutamylcysteine synthetase activity in rat lung epithelial L2 cells. J Biol Chem. 1994 Oct 21;269(42):26512-7.

Shi MM, Iwamoto T, Forman HJ. gamma-Glutamylcysteine synthetase and GSH increase in quinone-induced oxidative stress in BPAEC. Am J Physiol. 1994 Oct;267(4 Pt 1):L414-21.

Rajagopalan, S., Heck, T. J., Iwamoto, T. and Tomich, J. M.  (1995)  Use of the 3-nitro-2-pyridine sulfenyl protecting group to introduce Ne-branching at lysine during solid-phase peptide synthesis:  I. Application to the synthesis of a peptide template containing two addressable sites.  Intl. J. Prot. Pept. Res. 45, 173-179.

Forman HJ, Shi MM, Iwamoto T, Liu RM, Robison TW. Measurement of gamma-glutamyl transpeptidase and gamma-glutamylcysteine synthetase activities in cells. Methods Enzymol. 1995;252:66-71

Pandit, S. D., Donis-Keller, H., Iwamoto, T., Tomich, J. M., Pike, L. J. (1996) The MEN 2B point mutation alters the transforming capacity of the EGF receptor. J. Biol. Chem. 271, 5850-5858.

Wallace, D. P., Tomich, J. M., Iwamoto, T., Henderson, K., Grantham, J. J. (1997) and Sullivan, L. P. A synthetic peptide derived from the glycine-gated Cl- channel generates Cl- and fluid secretion by epithelial monolayers.  Am. J. Physiol: 272 (Cell Physiol. 41) C1672-C1679.

Tomich, J. M., Wallace, D. P., Henderson, K., Brandt, R., Scott, A. J., Mitchell, K. E. Radke, G.,Grantham, J. J.  Sullivan, L. P. & Iwamoto, T. (1998) Aqueous solubilization of transmembrane peptide sequences with retention of membrane insertion and function. Biophys J.  74, 256-267.

Wallace, D. P., Tomich, J. M., Eppler, J., Iwamoto, T., Grantham, J. J.and Sullivan, (2000)  L. P. A Channel forming peptide induces Cl- secretion by T84 cells: Modulation by  Ca2+ - dependent K+ channels. Biochim. Biophys. Acta  1464, 69-82. 

Mitchell, K. E., Tomich, J. M., Iwamoto, T. and Freeman, L.C.(2000) A synthetic peptide based on a glycine-gated chloride channel induces a novel chloride conductance in isolated epithelial cells. Biochim. Biophys. Acta  1466, 47-60.

Broughman, J.R., Mitchell, K., Iwamoto, T., Tomich J.M. and Schultz, B.D. (2001)  Amino-terminal modification of a channel-forming peptide increases capacity for epithelia anion secretion. Am. J. Physiol. (Cell) 280, C451-C458.

Gao, L., Broughman, J.R., Iwamoto, T., Tomich, J.M., Venglarik, C.J.and Forman, H.J. (2001) Synthetic Cl- channel restores glutathione secretion in airway epithelia. Am.J.Physiol  (Lung) 281 L24-L30.

Broughman JR, Shank LP, Takeguchi W, Iwamoto T, Mitchell KE, Schultz, BD and Tomich JM. (2002) Distinct structural elements that direct solution aggregation and membrane assembly in the channel forming peptide M2GlyR. Biochemistry  41,7350-7358.

Broughman JR, Shank LP, Iwamoto T, Prakash O, Schultz BD, Tomich JM and Mitchell KE (2002) Structural implications of placing cationic residues at either the NH2- or COOH- terminus in a pore-forming synthetic peptide. J Membrane Biol. 190: 93-103.

Dickinson DA, Iles KE, Watanabe N, Iwamoto T, Zhang H, Krzywanski DM, Forman HJ.(2002) 4-hydroxynonenal induces glutamate cysteine ligase through JNK in HBE1 cells. Free Radic Biol Med. 33:974.

Watanabe N, Iwamoto T, Dickinson DA, Iles KE, Forman HJ. (2002)  Activation of the mitochondrial caspase cascade in the absence of protein synthesis does not require c-Jun N-terminal kinase. Arch Biochem Biophys. 405(2):231-40.

Iles KE, Dickinson DA, Watanabe N, Iwamoto T, Forman HJ. (2002) AP-1 activation through endogenous H(2)O(2) generation by alveolar macrophages. Free Radic Biol Med 32(12):1304-13. Erratum in: Free Radic Biol Med. 33(5):725.

Matsumoto N, Tsuruoka S, Iwamoto T, Tomich JM, Ito K, Imai M and Suzuki M. (2003)  Alterations in Ion Transport Induced by an Artificial Cl- Channel in Micro-Perfused Renal Proximal Tubules.   J. Membr. Biol. 193:195-200.

Watanabe N, Iwamoto T, Bowen KD, Dickinson DA, Torres M, Forman HJ. (2003) Bio-effectiveness of Tat-catalase conjugate: a potential tool for the identification of H2O2-dependent cellular signal transduction pathways. Biochem Biophys Res Commun. 303(1):287-93.

Cook GA, Prakash O, Zhang K, Shank LP, Robbins, A, Gong Y-X, Iwamoto T, Schultz, BD and Tomich JM. (2004) Activity and Structural Comparisons of Solution Associating and Monomeric Channel-Forming Peptides Derived from the Glycine Receptor M2 Segment. Biophysical J. 86: 1424-1435.

Broughman JR, Brandt, R., Hastings C, Iwamoto T, Tomich JM and Schultz BD, (2004)   Anion Channel-Forming Peptide Modulates Transepithelial Electrical Conductance and Solute Permeability. Am. J. Physiol:  (Cell Physiol. ) 286: C1312-1323.

Iwamoto T, You M, Li E, Spangler J, Tomich JM, Hristova K  (2005) Synthesis And Initial Characterization Of Fgfr3 Transmembrane Domain: Consequences Of Sequence Modifications. In Press BBA-Biomembranes

Broughman JR, Brandt, R., Hastings C, Iwamoto T, Tomich JM and Schultz BD, (2004) Anion Channel Forming Peptide Modulates Transepithelial Electrical Conductance and Solute Permeability. Am. J. Physiol: (Cell Physiol.) 286: C1312-1323

Cook GA, Prakash O, Zhang K, Shank LP, Robbins, A,, Gong Y-X, Iwamoto T, Schultz, BD and Tomich JM. (2004) Activity and Structural Comparisons of Solution Associating and Monomeric Channel-Forming Peptides Derived from the Glycine Receptor M2 Segment. Biophysical J. 86: 1424-1435.

No Previous or Current Funding